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journal article·2016-11-01Open access

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

S. Caponi, S. Mattana, M. Ricci, K. Sagini, L. J. Juarez-Hernandez, A. M. Jimenez-Garduño, N. Cornella, L. Pasquardini, L. Urbanelli, P. Sassi, A. Morresi, C. Emiliani, D. Fioretto, M. Dalla Serra, C. Pederzolli, S. Iannotta, P. Macchi, C. Musio

AIP Advances· Volume 6· Issue 11
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Abstract

A living bio-hybrid system has been successfully implemented. It is constituted by neuroblastic cells, the SH-SY5Y human neuroblastoma cells, adhering to a poly-anyline (PANI) a semiconductor polymer with memristive properties. By a multidisciplinary approach, the biocompatibility of the substrate has been analyzed and the functionality of the adhering cells has been investigated. We found that the PANI films can support the cell adhesion. Moreover, the SH-SY5Y cells were successfully differentiated into neuron-like cells for in vitro applications demonstrating that PANI can also promote cell differentiation. In order to deeply characterize the modifications of the bio-functionality induced by the cell-substrate interaction, the functional properties of the cells have been characterized by electrophysiology and Raman spectroscopy. Our results confirm that the PANI films do not strongly affect the general properties of the cells, ensuring their viability without toxic effects on their physiology. Ascribed to the adhesion process, however, a slight increase of the markers of the cell suffering has been evidenced by Raman spectroscopy and accordingly the electrophysiology shows a reduction at positive stimulations in the cells excitability.
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Research context

PolymersBiomaterialsElectronic materials

OpenAlex topics: Neuroscience and Neural Engineering · Advanced Memory and Neural Computing · Photoreceptor and optogenetics research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Unità di Perugia 1 Istituto Officina dei Materiali del CNR (CNR-IOM), , c/o Dipartimento di Fisica e Geologia, Via Pascoli, Perugia, Italy

Università di Perugia,Via Elce di sotto 8 2 Dipartimento di Chimica, Biologia e Biotecnologie, , 06123 Perugia, Italy

Unità di Trento, and LabSSAH FBK 3 Istituto di Biofisica CNR (IBF-CNR), , Via Sommarive 18, 38123 Trento, Italy

Università di Trento 4 Centre for Integrative Biology (CIBIO), , Via delle Regole 101, 38123 Trento, Italy

University of Trento 5 Department of Industrial Engineering, , Via Sommarive 9, 38123 Povo (TN), Italy

Consiglio Nazionale delle Ricerche (IMEM-CNR) 6 Istituto dei Materiali per l’Elettronica ed il Magnetismo, , Parco Area delle Scienze 37/A, 43124 Parma, Italy

Università di Perugia 7 Centro di Eccellenza sui Materiali Innovativi Nanostrutturati (CEMIN), , Perugia, Italy

Università di Perugia 8 Dipartimento di Fisica e Geologia, , Via Pascoli, Perugia, Italy

Fondazione Bruno Kessler (FBK) 9 , Via Sommarive 18, 38123 Trento, Italy

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