journal article·2020-11-01Open access

Real-time detection of cardiac troponin I and mechanism analysis of AlGaAs/GaAs high electron mobility transistor biosensor

Jiaming Luo, Sufang Li, Mengke Xu, Min Guan, Mengxi Yang, Jingyi Ren, Yang Zhang, Yiping Zeng

AIP Advances· Volume 10· Issue 11

Abstract

The high electron mobility transistor (HEMT)-based biosensors are highly competitive in the ultimate application of portable and point-of-care testing. Herein, we have demonstrated highly sensitive and real-time detection of cardiac troponin I (cTnI), a biomarker for the diagnosis of acute myocardial infarction (AMI) using AlGaAs/GaAs HEMT-based biosensors. The device has achieved a lower detection limit of 1 pg/ml in the buffer solution and less than 30 s response time, which demonstrated significant promise in the early diagnosis and screening of AMI. In addition, our results are consistent with the enzyme-linked immunosorbent assay according to the AMI patient’s blood test results. Furthermore, by comparing the two HEMT structures, we also calculated the equilibrium dissociation constant (KD) of the cTnI and cTnI antibody and analyzed the sensing mechanism. The results show that this method is very promising for early diagnosis of AMI.
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Research context

OpenAlex topics: Nanowire Synthesis and Applications · Analytical Chemistry and Sensors · Semiconductor materials and devices

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory of Semiconductor Material Sciences, Beijing Key Laboratory of Low Dimensional Semiconductor Materials and Devices, Institute of Semiconductors, Chinese Academy of Sciences 1 , Beijing 100083, People’s Republic of China

College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences 2 , Beijing 100049, China

Department of Cardiology, Peking University People’s Hospital 3 , Beijing 100044, People’s Republic of China

China-Japan Friendship Hospital 4 , Beijing 100029, People’s Republic of China

Funding

The National Key R&D Program of China · the Capital Health Research and Development of Special · the National Natural Science Foundation of China · the Youth Innovation Promotion Association CAS

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