Impact of Vitamin D3 Functionalization on the Osteogenic Capacity of Bioinspired 3D Scaffolds Based on Ce-Doped Bioactive Glass and Spongia Agaricina
Source abstract
Abstract
Reconstruction of extensive bone defects due to age, trauma, or post-illness conditions remains challenging. Biomimetic scaffolds with osteogenic capabilities have been proposed as an alternative to the classical autograft and allograft implants. Three-dimensional scaffolds were obtained based on Ce-doped mesoporous bioactive glass (MBG) and Spongia agaricina (SA) as sacrificial templates functionalized with vitamin D3. The study aimed to investigate the effect of vitamin D3 functionalization on the optimal variant of a 3D scaffold doped with 3 mol% ceria, selected in our previous work based on its biological and physicochemical properties. Scanning electron microscopy (SEM) images of the non-functionalized/functionalized scaffolds revealed a porous structure with interconnected pores ranging from 100 to 350 μm. Fourier transform infrared spectroscopy (FTIR) and SEM analysis confirmed the surface functionalization. Cytotoxicity evaluation showed that all investigated scaffolds do not exhibit cytotoxicity and genotoxicity toward the Saos-2 osteosarcoma cell line. Moreover, the study demonstrated that functionalization with vitamin D3 enhanced osteogenic activity in dental pulp stem cells (DPSCs) by increasing calcium deposition and osteocalcin secretion, as determined by Alizarin red stain and a colorimetric ELISA kit, as a result of its synergistic action with cerium ions. The results showed that the Ce-doped MBG scaffold functionalized with vitamin D3 had the potential for applications in bone regeneration.
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Research context
OpenAlex topics: Bone Tissue Engineering Materials · Layered Double Hydroxides Synthesis and Applications · biodegradable polymer synthesis and properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
National Institute of Research and Development for Biological Sciences, 296, Spl. Independentei, 060031 Bucharest, Romania
“Ilie Murgulescu” Institute of the Physical Chemistry of the Romanian Academy, 202, Spl. Independentei, 060021 Bucharest, Romania
Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology Politehnica of Bucharest, 060042 Bucharest, Romania
Funding
Program Nucleu within the National Plan for Research, Development, and Innovation 2022–2027, implemented with the support of the Ministry of Education and Research · Executive Agency for Higher Education, Research, Development and Innovation Funding (UEFISCDI)
References (46)
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