Characteristics and Antitumor Activity of Doxorubicin-Loaded Multifunctional Iron Oxide Nanoparticles in MEC1 and RM1 Cell Lines
Source abstract
Abstract
The rapid progress in nanotechnology has introduced multifunctional iron oxide nanoparticles as promising agents in cancer treatment. This research focused on the synthesis and assessment of citric-acid-coated, folic-acid-conjugated nanoparticles loaded with doxorubicin, evaluating their therapeutic potential in tumor models. An advanced automated continuous technology line (CTL) utilizing a controlled co-precipitation method was employed to produce highly dispersive, multifunctional nanofluids with a narrow size distribution. Various techniques, including dynamic light scattering (DLS), electrophoretic light scattering (ELS), X-ray diffraction (XRD), and transmission electron microscopy (TEM), were employed to examine the particle size, zeta potential, structure, and morphology. Magnetic properties were analyzed through vibrating sample magnetometry (VSM), and surface modifications were confirmed via UV-visible (UV-Vis) and Fourier-Transform Infrared (FTIR) spectroscopy. Cytotoxicity and drug delivery efficiency were evaluated in vitro using RM1 (prostate cancer) and MEC1 (chronic lymphocytic leukemia) cell lines. Fluorescence microscopy demonstrated the successful intracellular delivery of doxorubicin, showcasing the nanoparticles’ potential for targeted cancer therapy. However, folic-acid-conjugated nanoparticles exhibited diminished effectiveness over time. This study highlights the importance of nanoparticle optimization for enhancing therapeutic performance. Further research should aim to improve nanoparticle formulations and explore their long-term impacts for the development of safe, targeted cancer treatments.
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Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Iron oxide chemistry and applications · Characterization and Applications of Magnetic Nanoparticles
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Division of Immunology and Microbiology, Iv. Javakhishvili Tbilisi State University, 1, Ilia Tchavchavadze Ave., 0179 Tbilisi, Georgia
Nanocomposites Laboratory, Vladimer Chavchanidze Institute of Cybernetics of the Georgian Technical University, Z. Anjafaridze Str. 5, 0186 Tbilisi, Georgia
Institute for Technical Physics and Materials Science, HUN-REN Centre for Energy Research, Konkoly-Thege Miklós Str. 29-33, 1121 Budapest, Hungary
Institute for Energy Security and Environmental Safety, HUN-REN Centre for Energy Research, Konkoly-Thege Miklós Str. 29-33, 1121 Budapest, Hungary
Funding
Shota Rustaveli National Science Foundation of Georgia (SRNSFG)
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