Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study
Source abstract
Abstract
This study evaluated the color change (ΔE00) and penetration depth (PD) of white spot lesions (WSLs) infiltrated with the resin infiltrant (Icon®) functionalized with methacrylate epigallocatechin-3-gallate (EGCG). To introduce polymerizable double bonds, EGCG was reacted with methacryloyl chloride (EM). Subsequently, the Icon resin infiltrant (I) was loaded with neat EGCG (IE) or EGCG–methacrylate (IEM) at 2 wt% each. WSLs were created on bovine enamel blocks and treated with I, IE, or IEM. Sound and untreated enamel surfaces were used as controls (C). Infiltrant PD (%) was determined by Confocal Laser Scanning Microscopy (CLSM, n = 12) analysis. For color change (ΔE00) determination (n = 14), ΔL, Δa, and Δb, half of each sample was kept sound as a reference area. The color was determined with a spectrophotometer. Data were statistically evaluated (p = 0.05). Surface morphology was obtained as a qualitative response variable using 3D CLSM. PD (%) did not differ statistically for I, IE, and IEM (p = 0.780). Groups I and IEM showed similar performance on color change (ΔE00) compared to the control group, while IE exhibited intermediate results, with no significant difference observed between the untreated, I, and IEM groups (p < 0.001). IEM promoted the masking of the WSL color without interfering with the PD.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Dental materials and restorations · Dental Erosion and Treatment · Temporomandibular Joint Disorders
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Restorative Discipline, Dental Research Institute, Faculty of Dentistry, University of Toronto, 101 Elm Street, Toronto, ON M5G 1G6, Canada
Department of Operative Dentistry, School of Dentistry, University of São Paulo, Avenida Professor Lineu Prestes, 2227, São Paulo 05508-000, SP, Brazil
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, Bauru 17012-901, SP, Brazil
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, Bauru 17012-901, SP, Brazil
Department of Restorative and Prosthetic Dental Sciences, College of Dentistry, King Saud bin Abdulaziz University for Health Sciences, P.O. Box 3660, Riyadh 11481 K.S.A, Saudi Arabia
Funding
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES) · Programa Institucional de Internacionalização da Universidade de São Paulo em convênio com a CAPES · Natural Sciences and Engineering Research Council of Canada
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