3D Bioprinting of Smart Oxygen-Releasing Cartilage Scaffolds
Source abstract
Abstract
Three-dimensional bioprinting is a powerful technique for manufacturing improved engineered tissues. Three-dimensional bioprinted hydrogels have significantly advanced the medical field to repair cartilage tissue, allowing for such constructs to be loaded with different components, such as cells, nanoparticles, and/or drugs. Cartilage, as an avascular tissue, presents extreme difficulty in self-repair when it has been damaged. In this way, hydrogels with optimal chemical and physical properties have been researched to respond to external stimuli and release various bioactive agents to further promote a desired tissue response. For instance, methacryloyl gelatin (GelMA) is a type of modified hydrogel that allows for the encapsulation of cells, as well as oxygen-releasing nanoparticles that, in the presence of an aqueous medium and through controlled porosity and swelling, allow for internal and external environmental exchanges. This review explores the 3D bioprinting of hydrogels, with a particular focus on GelMA hydrogels, to repair cartilage tissue. Recent advances and future perspectives are described.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: 3D Printing in Biomedical Research · Osteoarthritis Treatment and Mechanisms · Silk-based biomaterials and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Faculty of Medical Science, State University of Campinas, Campinas 13083-872, SP, Brazil
School of Biological Sciences and Engineering, Yachay Tech University, Urcuquí 100650, Ecuador
Interdisciplinary Laboratory for Advanced Materials (LIMAV), Materials Science and Engineering Graduate Program (PPGCM), Federal University of Piaui (UFPI), Teresina 64049-550, PI, Brazil
Bioengineering Program, Scientific and Technological Institute, Brasil University, São Paulo 13566-590, SP, Brazil
Department of Physics, UFPI—Federal University of Piaui, Teresina 64049-550, PI, Brazil
School of Engineering, Saveetha University, Chennai 602117, India
School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 065000, China
Funding
National Council for Scientific and Technological Development
References (85)
Show source-provided references
1. The Role of Metabolism in the Pathogenesis of Osteoarthritis10.1038/nrrheum.2017.50 ↗
2. Cartilage Tissue Engineering: Controversy in the Effect of Oxygen10.1080/bty.23.3.175 ↗
3. Huang, H., Xu, H., and Zhang, J. (2019). Current Tissue Engineering Approaches for Cartilage Regeneration. Cartilage Tissue Engineering and Regeneration Techniques, IntechOpen.10.5772/intechopen.84429 ↗
4. 3D Bioprinting for the Repair of Articular Cartilage and Osteochondral Tissue10.1016/j.bprint.2022.e00239 ↗
5. Breathing Life into Engineered Tissues Using Oxygen-Releasing Biomaterials10.1038/s41427-019-0166-2 ↗
6. Londono, R., Wenzhong, W., Wang, B., Tuan, R.S., and Lozito, T.P. (2017). Cartilage and Muscle Cell Fate and Origins during Lizard Tail Regeneration. Front. Bioeng. Biotechnol., 5.10.3389/fbioe.2017.00070 ↗
7. Kaoud, H.A.E.-S. (2018). Introductory Chapter: Concepts of Tissue Regeneration. Tissue Regeneration, IntechOpen.10.5772/intechopen.76996 ↗
8. Sánchez-Téllez, D., Téllez-Jurado, L., and Rodríguez-Lorenzo, L. (2017). Hydrogels for Cartilage Regeneration, from Polysaccharides to Hybrids. Polymers, 9.10.3390/polym9120671 ↗
9. Oxygen-Releasing Biomaterials for Tissue Engineering10.1002/pi.4502 ↗
10. Nonvasoconstrictive Hemoglobin Particles as Oxygen Carriers10.1021/nn402073n ↗
11. Ward, C.L., Corona, B.T., Yoo, J.J., Harrison, B.S., and Christ, G.J. (2013). Oxygen Generating Biomaterials Preserve Skeletal Muscle Homeostasis under Hypoxic and Ischemic Conditions. PLoS ONE, 8.10.1371/journal.pone.0072485 ↗
12. Fabrication of Amine-decorated Nonspherical Microparticles with Calcium Peroxide Cargo for Controlled Release of Oxygen10.1002/jbm.a.36799 ↗
13. An Oxygen Release System to Augment Cardiac Progenitor Cell Survival and Differentiation under Hypoxic Condition10.1016/j.biomaterials.2012.05.012 ↗
14. Oxygen and Nutrient Delivery in Tissue Engineering: Approaches to Graft Vascularization10.1002/term.2932 ↗
15. Measurement of PH and Ionic Composition of Pericellular Sites10.1098/rstb.1975.0050 ↗
16. Bovine Articular Chondrocyte Function in Vitro Depends upon Oxygen Tension10.1053/joca.1999.0314 ↗
17. HIF-1 and P53: Communication of Transcription Factors under Hypoxia10.1111/j.1582-4934.2004.tb00467.x ↗
18. Oxygen-Generating Biomaterials: A New, Viable Paradigm for Tissue Engineering?10.1016/j.tibtech.2016.05.012 ↗
19. Advances in Controlled Oxygen Generating Biomaterials for Tissue Engineering and Regenerative Therapy10.1021/acs.biomac.9b00546 ↗
20. Nanocomposite Hydrogels for Cartilage Tissue Engineering: A Review10.1080/21691401.2017.1345924 ↗
21. Nanoparticle-Hydrogel Composites: Concept, Design, and Applications of These Promising, Multi-Functional Materials10.1002/advs.201400010 ↗
22. Engineering Calcium Peroxide Based Oxygen Generating Scaffolds for Tissue Survival10.1039/D0BM02048F ↗
23. Oxygen-Generating Photo-Cross-Linkable Hydrogels Support Cardiac Progenitor Cell Survival by Reducing Hypoxia-Induced Necrosis10.1021/acsbiomaterials.6b00109 ↗
24. An Enzyme-Modulated Oxygen-Producing Micro-System for Regenerative Therapeutics10.1016/j.ijpharm.2011.02.041 ↗
25. Choi, J., Hong, G., Kwon, T., and Lim, J. (2018). Fabrication of Oxygen Releasing Scaffold by Embedding H2O2-PLGA Microspheres into Alginate-Based Hydrogel Sponge and Its Application for Wound Healing. Appl. Sci., 8.10.3390/app8091492 ↗
26. West, H., Roberts, F., Sweeney, P., Walker-Samuel, S., Leedale, J., Colley, H., Murdoch, C., Shipley, R.J., and Webb, S. (2021). A Mathematical Investigation into the Uptake Kinetics of Nanoparticles in Vitro. PLoS ONE, 16.10.1371/journal.pone.0254208 ↗
27. Oxygen-Generating Hybrid Polymeric Nanoparticles with Encapsulated Doxorubicin and Chlorin E6 for Trimodal Imaging-Guided Combined Chemo-Photodynamic Therapy10.7150/thno.22989 ↗
28. Oxygen-Releasing Biomaterials: Current Challenges and Future Applications10.1016/j.tibtech.2021.01.007 ↗
29. Drug Release from Hydrogel Matrices
30. Oxygen Delivering Biomaterials for Tissue Engineering10.1039/C5TB02635K ↗
31. Bioprinting Technology and Its Applications10.1093/ejcts/ezu148 ↗
32. 3D Printing of Porous Hydroxyapatite Scaffolds Intended for Use in Bone Tissue Engineering Applications10.1016/j.msec.2014.11.024 ↗
33. Bioprinting Toward Organ Fabrication: Challenges and Future Trends10.1109/TBME.2013.2243912 ↗
34. Tissue and Organ 3D Bioprinting10.1177/2472630318760515 ↗
35. Tarassoli, S.P., Jessop, Z.M., Jovic, T., Hawkins, K., and Whitaker, I.S. (2021). Candidate Bioinks for Extrusion 3D Bioprinting—A Systematic Review of the Literature. Front. Bioeng. Biotechnol., 9.10.3389/fbioe.2021.616753 ↗
36. Bioprinting Technology: A Current State-of-the-Art Review10.1115/1.4028512 ↗
37. Third-Dimensional Organ and Tissue Printing: Techniques, Applications, and Limitations
38. Smart Hydrogels for 3D Bioprinting
39. Additive Manufacturing of Tissues and Organs10.1016/j.progpolymsci.2011.11.007 ↗
40. Biofabrication of Multi-Material Anatomically Shaped Tissue Constructs10.1088/1758-5082/5/3/035007 ↗
41. Complex Heterogeneous Tissue Constructs Containing Multiple Cell Types Prepared by Inkjet Printing Technology10.1016/j.biomaterials.2012.09.035 ↗
42. Michael, S., Sorg, H., Peck, C.-T., Koch, L., Deiwick, A., Chichkov, B., Vogt, P.M., and Reimers, K. (2013). Tissue Engineered Skin Substitutes Created by Laser-Assisted Bioprinting Form Skin-Like Structures in the Dorsal Skin Fold Chamber in Mice. PLoS ONE, 8.10.1371/journal.pone.0057741 ↗
43. Digital Microfabrication of User-Defined 3D Microstructures in Cell-Laden Hydrogels10.1002/bit.24957 ↗
44. Vertex Dynamics Simulations of Viscosity-Dependent Deformation during Tissue Morphogenesis10.1007/s10237-014-0613-5 ↗
45. The 3D Printing of Gelatin Methacrylamide Cell-Laden Tissue-Engineered Constructs with High Cell Viability10.1016/j.biomaterials.2013.09.078 ↗
46. Hafeez, S., Ooi, H., Morgan, F., Mota, C., Dettin, M., van Blitterswijk, C., Moroni, L., and Baker, M. (2018). Viscoelastic Oxidized Alginates with Reversible Imine Type Crosslinks: Self-Healing, Injectable, and Bioprintable Hydrogels. Gels, 4.10.3390/gels4040085 ↗
47. Microscale Tissue Engineering Using Gravity-Enforced Cell Assembly10.1016/j.tibtech.2004.02.002 ↗
48. 3D Bioprinting of Low-Concentration Cell-Laden Gelatin Methacrylate (GelMA) Bioinks with a Two-Step Cross-Linking Strategy10.1021/acsami.7b16059 ↗
49. 3D Bioprinting of Functional Tissue Models for Personalized Drug Screening and in Vitro Disease Modeling10.1016/j.addr.2018.06.011 ↗
50. Biomimetic Hydrogels Designed for Cartilage Tissue Engineering10.1039/D0BM01852J ↗
51. A Review on Some Natural Biopolymers and Their Applications in Angiogenesis and Tissue Engineering10.29252/JABR.05.03.01 ↗
52. Overview of Natural Hydrogels for Regenerative Medicine Applications10.1007/s10856-019-6318-7 ↗
53. Hyaluronan Hydrogels with a Low Degree of Modification as Scaffolds for Cartilage Engineering10.1016/j.ijbiomac.2017.05.091 ↗
54. Natural Hydrogels for Cartilage Regeneration: Modification, Preparation and Application
55. An Injectable Collagen-Genipin-Carbon Dot Hydrogel Combined with Photodynamic Therapy to Enhance Chondrogenesis10.1016/j.biomaterials.2019.05.001 ↗
56. Fibrin Hydrogels Functionalized with Cartilage Extracellular Matrix and Incorporating Freshly Isolated Stromal Cells as an Injectable for Cartilage Regeneration10.1016/j.actbio.2016.03.008 ↗
57. Gelatin-Based Hydrogels as Potential Biomaterials for Colonic Delivery of Oxaliplatin10.1016/j.ijpharm.2018.12.020 ↗
58. Functionalized Synthetic Biodegradable Polymer Scaffolds for Tissue Engineering10.1002/mabi.201100466 ↗
59. Biodegradable and Biocompatible Polymers for Tissue Engineering Application: A Review10.3109/21691401.2016.1146731 ↗
60. Advances of Injectable Hydrogel-Based Scaffolds for Cartilage Regeneration10.1093/rb/rbz022 ↗
61. Sun, M., Sun, X., Wang, Z., Guo, S., Yu, G., and Yang, H. (2018). Synthesis and Properties of Gelatin Methacryloyl (GelMA) Hydrogels and Their Recent Applications in Load-Bearing Tissue. Polymers, 10.10.3390/polym10111290 ↗
62. Wang, H., Zhou, H., Liu, H., Yuan, T., and Li, X. (2015, January 28–29). Controlled-Release Oxygen Using Polymers. Proceedings of the 2015 6th International Conference on Manufacturing Science and Engineering, Guangzhou, China.10.2991/icmse-15.2015.309 ↗
63. Oh, S.H., Ward, C.L., Atala, A., Yoo, J.J., and Harrison, B.S. (2009). Oxygen Generating Scaffolds for Enhancing Engineered Tissue Survival. Biomaterials.10.1016/j.biomaterials.2008.09.065 ↗
64. Direct Human Cartilage Repair Using Three-Dimensional Bioprinting Technology10.1089/ten.tea.2011.0543 ↗
65. Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs10.1002/mabi.201200471 ↗
66. 3D Bioprinting via an in Situ Crosslinking Technique towards Engineering Cartilage Tissue10.1038/s41598-019-56117-3 ↗
67. Barbon, S., Contran, M., Stocco, E., Todros, S., Macchi, V., Caro, R.D., and Porzionato, A. (2021). Enhanced Biomechanical Properties of Polyvinyl Alcohol-Based Hybrid Scaffolds for Cartilage Tissue Engineering. Processes, 9.10.3390/pr9050730 ↗
68. Gelatin Methacrylate (GelMA)-Based Hydrogels for Cell Transplantation: An Effective Strategy for Tissue Engineering10.1007/s12015-019-09893-4 ↗
69. Naranda, J., Bračič, M., Vogrin, M., and Maver, U. (2021). Recent Advancements in 3D Printing of Polysaccharide Hydrogels in Cartilage Tissue Engineering. Materials, 14.10.3390/ma14143977 ↗
70. Gelatin Hydrogels Formed by Orthogonal Thiol–Norbornene Photochemistry for Cell Encapsulation10.1039/C4BM00070F ↗
71. Synthesis, Properties, and Biomedical Applications of Gelatin Methacryloyl (GelMA) Hydrogels10.1016/j.biomaterials.2015.08.045 ↗
72. Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering10.1002/adhm.201500005 ↗
73. Gelatin Methacrylate Scaffold for Bone Tissue Engineering: The Influence of Polymer Concentration10.1002/jbm.a.36226 ↗
74. Photopolymerization of Cell-Laden Gelatin Methacryloyl Hydrogels Using a Dental Curing Light for Regenerative Dentistry10.1016/j.dental.2017.11.020 ↗
75. Lee, B., Lum, N., Seow, L., Lim, P., and Tan, L. (2016). Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications. Materials, 9.10.3390/ma9100797 ↗
76. You, F., Eames, B.F., and Chen, X. (2017). Application of Extrusion-Based Hydrogel Bioprinting for Cartilage Tissue Engineering. Int. J. Mol. Sci., 18.10.3390/ijms18071597 ↗
77. 3D Bioprinting of Spatially Heterogeneous Collagen Constructs for Cartilage Tissue Engineering10.1021/acsbiomaterials.6b00288 ↗
78. Hybrid Printing of Mechanically and Biologically Improved Constructs for Cartilage Tissue Engineering Applications10.1088/1758-5082/5/1/015001 ↗
79. Three-Dimensional Bioprinting in Tissue Engineering and Regenerative Medicine10.1007/s10529-015-1975-1 ↗
80. Bio-Printing of Collagen and VEGF-Releasing Fibrin Gel Scaffolds for Neural Stem Cell Culture10.1016/j.expneurol.2010.02.014 ↗
81. Omnidirectional Printing of 3D Microvascular Networks
82. Rapid 3D Printing of Anatomically Accurate and Mechanically Heterogeneous Aortic Valve Hydrogel Scaffolds10.1088/1758-5082/4/3/035005 ↗
83. The Effect of Mean Pore Size on Cell Attachment, Proliferation and Migration in Collagen–Glycosaminoglycan Scaffolds for Bone Tissue Engineering10.1016/j.biomaterials.2009.09.063 ↗
84. Biofabrication of Natural Hydrogels for Cardiac, Neural, and Bone Tissue Engineering Applications
85. Biomedical Application of Photo-Crosslinked Gelatin Hydrogels10.1186/s42825-020-00043-y ↗