Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration
Source abstract
Abstract
Cell migration of mesenchymal stem cells (MSCs) is critical for bone healing and remodeling. Cobalt is a well-known hypoxia mimic, which can enhance MSC migration. Therefore, the objective of this study was to investigate the migratory response of MSCs to a developed cobalt-incorporated hydroxyapatite (HACo) material. HACo was fabricated by a simple ion exchange procedure at concentrations ranging from 40 to 8000 μM into disc shape. HACo discs were incubated in the media and conditioned media (CM; HACoCM) were collected for MSC culture. HACM served as a control. MSCs were cultured until reaching 90% confluence before the wound was generated by scraping. Time-lapse imaging of wound migration was monitored, recorded, and assessed. Statistical analysis was performed by one-way ANOVA followed by a Dunnett’s test. The wound area gradually declined from 0 to 40 h for all samples. HACoCM at 40 µM (HACo40CM) promoted wound closure at the early period of wound healing. Both HACo40CM and HACo8000CM enhanced the distance and velocity of individual cell migration. However, only HACo40CM affected cell persistence and direction at the early period of cell migration. Exposure to HACoCM accelerated the speed of MSC migration, which is necessary for wound healing. The migratory ability of individual cells could help the rate of wound healing. Therefore, HACo materials may serve as potential biomaterials for enhanced bone healing.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Mesenchymal stem cell research · Bone Tissue Engineering Materials · Wound Healing and Treatments
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Implantology, Faculty of Dentistry, Thammasat University, Pathum Thani 12120, Thailand
Faculty of Dentistry, Thammasat University, Pathum Thani 12120, Thailand
Biofunctional Materials and Devices Research Group, National Metal and Materials Technology Center (MTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand
Thammasat University Center of Excellence in Computational Mechanics and Medical Engineering, Thammasat University, Pathum Thani 12120, Thailand
Thammasat University Research Unit in Dental and Bone Substitute Biomaterials, Faculty of Dentistry, Thammasat University, Pathum Thani 12120, Thailand
Funding
Thailand Science Research and Innovation Fundamental Fund
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