Formulative Study and Characterization of Novel Biomaterials Based on Chitosan/Hydrolyzed Collagen Films
Source abstract
Abstract
To date, the need for biomaterials capable of improving the treatment of chronic skin wounds remains a clinical challenge. The aim of the present work is to formulate and characterize chitosan (Cs)/hydrolyzed collagen (HC) films as potential biomaterials with improved mechanical and hydration performances compared to single component formulations. Films were made by the solvent casting method, with or without glycerin and/or PEG1500 as plasticizers, resulting in a total of eight formulations. All films were characterized by their physico-chemical characteristics and their mechanical and hydration features. A full factorial design was also used to statistically assess the effect of HC concentration, type and concentration of plasticizers and their possible interactions on mechanical and swelling behaviors. Solid state characterization confirmed the hybrid nature of the films, with suggested electrostatic interactions between Cs and HC. Mechanical and swelling properties, along with the analysis of the experimental design, allowed the identification of formulations containing high HC concentration (2% w/v) and glycerin or glycerin/PEG1500 as more suitable candidates for skin wound treatment. Finally, viability assay of immortalized human keratinocytes (HaCaT) showed no statistical differences in cell survival compared to the complete culture medium, suggesting their potential as a promising tool for biomedical applications.
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Research context
OpenAlex topics: Wound Healing and Treatments · Surgical Sutures and Adhesives · Collagen: Extraction and Characterization
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pharmacy and Pharmaceutical Technology, Cartuja Campus, University of Granada, 18071 Granada, Spain
Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy
Department of Histology, Institute of Neurosciences, Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain
Nanoscopy-UGR Laboratory, Department of Physical Chemistry, Unidad de Excelencia en Química Aplicada a Biomedicina y Medioambiente UEQ, Cartuja Campus, University of Granada, 18071 Granada, Spain
Instituto Andaluz de Ciencias de la Tierra, CSIC-University of Granada, 18100 Armilla, Spain
Department of Applied Physics, Faculty of Science, University of Granada, 18071 Granada, Spain
Funding
MCIN/AEI/10.13039/501100011033
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