Magnetic graphene oxide nanoparticles boost the neuronal differentiation of neural progenitor cells
Source abstract
Abstract
Post-central nervous system injury, the endogenous repair and mobilization of neural stem cells are insufficient, necessitating the reliance on exogenous cell transplantation as the predominant repair and replacement strategy. The behavior and differentiation fate of induced pluripotent stem cells (iPSCs) are highly susceptible to external stimuli, including the cell culture matrix and physical electromagnetic signals. In this study, we innovatively utilize magnetic graphene oxide composite nanoparticles to regulate the proliferation and neural lineage differentiation of induced pluripotent stem cell-derived neural progenitor cells (iPSC-derived hNPCs). Our results reveal that a specific concentration of magnetic graphene oxide effectively maintains stemness properties, promotes cell proliferation, and preferentially directs differentiation toward neuronal lineages under induced differentiation conditions. Additionally, this treatment upregulated the expression of synaptic-related proteins while concurrently reducing the astrocytic differentiation ratio. These findings provide a novel materials-based strategy for optimizing hNPCs in vitro culture and directed differentiation systems.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Rehabilitation Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510180, China
Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China
Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China
School of Medicine, South China University of Technology, Guangzhou 510180, China
Department of Rehabilitation Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, No. 106 Zhongshan Road II, Guangzhou 510080, China
Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Department of Histoembryology and Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
Funding
National Natural Science Foundation of China · Guangzhou Municipal Science and Technology Project · Natural Science Foundation of Guangdong Province
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