Personalized tissue-engineered veins – long term safety, functionality and cellular transcriptome analysis in large animals
Source abstract
Abstract
Tissue engineering is a promising methodology to produce advanced therapy medicinal products (ATMPs). We have developed personalized tissue engineered veins (P-TEV) as an alternative to autologous or synthetic vascular grafts utilized in reconstructive vein surgery. Our hypothesis is that individualization through reconditioning of a decellularized allogenic graft with autologous blood will prime the tissue for efficient recellularization, protect the graft from thrombosis, and decrease the risk of rejection. In this study, P-TEVs were transplanted to vena cava in pig, and the analysis of three veins after six months, six veins after 12 months and one vein after 14 months showed that all P-TEVs were fully patent, and the tissue was well recellularized and revascularized. To confirm that the ATMP product had the expected characteristics one year after transplantation, gene expression profiling of cells from P-TEV and native vena cava were analyzed and compared by qPCR and sequencing. The qPCR and bioinformatics analysis confirmed that the cells from the P-TEV were highly similar to the native cells, and we therefore conclude that P-TEV is functional and safe in large animals and have high potential for use as a clinical transplant graft.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Tissue Engineering and Regenerative Medicine · Electrospun Nanofibers in Biomedical Applications · Mesenchymal stem cell research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Sahlgrenska Academy, Institution of Medicine, Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Blå Stråket 5 B, 41345 Gothenburg, Sweden
RISE Research Institutes of Sweden, Methodology, Textile and Medical Device, Brinellgatan 4, 50462 Borås, Sweden
VERIGRAFT AB, Arvid Wallgrensbacke 20, 41346, Göteborg, Sweden
Systems Biology Research Center, School of Bioscience, University of Skövde, 54128 Skövde, Sweden
Gothenburg University, Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, 41345 Gothenburg, Sweden
RISE Research Institutes of Sweden, Agriculture and Food, Box 5401, 40229 Gothenburg, Sweden
TATAA Biocenter AB, Gothenburg, Sweden
Jesús Usón Minimally Invasive Surgery Centre, Cáceres 10004, Spain
CIBER de Enfermedades Cardiovasculares, CIBER CV, Madrid 28029, Spain
RICORS-TERAV Network, ISCIII, Madrid 28029, Spain
Gothenburg University, Department of Laboratory Medicine, Institute of Biomedicine, Gothenburg, Sweden
Funding
VINNOVA · Västra Götalandsregionen
References (54)
Show source-provided references
1. S.Mendis , P.Puska and B.Norrving , Global atlas on cardiovascular disease prevention and control , World Health Organization in Collaboration with the World Heart Federation and the World Stroke Organization , Geneva , 2011
2. Ezzati · Nat. Rev. Cardiol. · 2015 · 12 · 50810.1038/nrcardio.2015.82 ↗
3. Onida · Phlebology · 2016 · 31 · 7410.1177/0268355516628359 ↗
4. De Maeseneer · Eur. J. Vasc. Endovasc. Surg. · 2022 · 63 · 18410.1016/j.ejvs.2021.12.024 ↗
5. Plate · Surgery · 1984 · 96 · 503
6. Eklof · Vasc. Med. · 1998 · 3 · 15710.1177/1358836X9800300210 ↗
7. Lozano · J. Trauma · 2009 · 67 · E118
8. Kleive · J. Vasc. Surg.: Venous Lymphatic Disord. · 2018 · 6 · 66
9. Madden · Ann. Vasc. Surg. · 2004 · 18 · 45310.1007/s10016-004-0055-0 ↗
10. Jara · Dig. Surg. · 2015 · 32 · 13510.1159/000370008 ↗
11. Jost · J. Vasc. Surg. · 2001 · 33 · 32010.1067/mva.2001.112805 ↗
12. Faulkner · Arch. Surg. · 1979 · 114 · 93910.1001/archsurg.1979.01370320071013 ↗
13. Kovalic · Eur. J. Vasc. Endovasc. Surg. · 2002 · 24 · 53310.1053/ejvs.2002.1710 ↗
14. Manduz · Int. J. Angiol. · 2008 · 17 · 3710.1055/s-0031-1278278 ↗
15. Schmidli · Eur. J. Vasc. Endovasc. Surg. · 2004 · 27 · 25110.1016/j.ejvs.2003.12.001 ↗
16. Håkansson · J. Tissue Eng. Regener. Med. · 2021 · 15 · 81810.1002/term.3233 ↗
17. Gilbert · Biomaterials · 2006 · 27 · 3675
18. Jenndahl · Regener. Ther. · 2022 · 21 · 33110.1016/j.reth.2022.08.005 ↗
19. Hosgood · Transplantation · 2011 · 92 · 73510.1097/TP.0b013e31822d4e04 ↗
20. Steen · Scand. Cardiovasc. J. · 2016 · 50 · 19310.3109/14017431.2016.1154598 ↗
21. Nicholson · Am. J. Transplant. · 2013 · 13 · 124610.1111/ajt.12179 ↗
22. Ellegaard · Scand. J. Lab. Anim. Sci. · 1995 · 22 · 239
23. Isenberg · Circ. Res. · 2006 · 98 · 2510.1161/01.RES.0000196867.12470.84 ↗
24. Lin · Int. J. Mol. Sci. · 2018 · 19 · 210110.3390/ijms19072101 ↗
25. Pashneh-Tala · Tissue Eng., Part B · 2016 · 22 · 6810.1089/ten.teb.2015.0100 ↗
26. Scott · Semin. Vasc. Surg. · 2007 · 20 · 15810.1053/j.semvascsurg.2007.07.007 ↗
27. Gui · Tissue Eng., Part A · 2009 · 15 · 266510.1089/ten.tea.2008.0526 ↗
28. Heng · Front. Cardiovasc. Med. · 2021 · 8 · 67758810.3389/fcvm.2021.677588 ↗
29. Mahara · Biomaterials · 2015 · 58 · 5410.1016/j.biomaterials.2015.04.031 ↗
30. Bai · J. Biomed. Mater. Res., Part B · 2020 · 108 · 241710.1002/jbm.b.34574 ↗
31. Dahan · Tissue Eng., Part A · 2017 · 23 · 6910.1089/ten.tea.2016.0126 ↗
32. Quint · Proc. Natl. Acad. Sci. U. S. A. · 2011 · 108 · 921410.1073/pnas.1019506108 ↗
33. Melchiorri · Tissue Eng., Part C · 2016 · 22 · 66310.1089/ten.tec.2015.0562 ↗
34. Kaushal · Nat. Med. · 2001 · 7 · 103510.1038/nm0901-1035 ↗
35. Nakayama · Regener. Med. · 2015 · 10 · 74510.2217/rme.15.45 ↗
36. Shi · Stem Cells Dev. · 2013 · 22 · 63110.1089/scd.2012.0313 ↗
37. Simsa · PLoS One · 2018 · 13 · e020926910.1371/journal.pone.0209269 ↗
38. Smith · Crit. Rev. Biochem. Mol. Biol. · 2015 · 50 · 32610.3109/10409238.2015.1050550 ↗
39. Ullah · Biomaterials · 2019 · 216 · 11928310.1016/j.biomaterials.2019.119283 ↗
40. Ikada · J. R. Soc., Interface · 2006 · 3 · 58910.1098/rsif.2006.0124 ↗
41. Crapo · Biomaterials · 2011 · 32 · 323310.1016/j.biomaterials.2011.01.057 ↗
42. Liu · Tissue Eng., Part B · 2018 · 24 · 13310.1089/ten.teb.2017.0350 ↗
43. Olive · Int. J. Radiat. Oncol., Biol., Phys. · 1992 · 22 · 39710.1016/0360-3016(92)90840-E ↗
44. Tannock · Br. J. Radiol. · 1972 · 45 · 51510.1259/0007-1285-45-535-515 ↗
45. Thomlinson · Br. J. Cancer · 1955 · 9 · 53910.1038/bjc.1955.55 ↗
46. Das · Cardiovasc. Intervention Radiol. · 2011 · 34 · 95810.1007/s00270-011-0096-z ↗
47. Simsa · PLoS One · 2019 · 14 · e022074310.1371/journal.pone.0220743 ↗
48. Untergasser · Nucleic Acids Res. · 2012 · 40 · e11510.1093/nar/gks596 ↗
49. Ye · BMC Bioinf. · 2012 · 13 · 13410.1186/1471-2105-13-134 ↗
50. Koressaar · Bioinformatics · 2007 · 23 · 128910.1093/bioinformatics/btm091 ↗
51. Kõressaar · Bioinformatics · 2018 · 34 · 193710.1093/bioinformatics/bty036 ↗
52. Bustin · Clin. Chem. · 2009 · 55 · 61110.1373/clinchem.2008.112797 ↗
53. Stuart · Cell · 2019 · 177 · 188810.1016/j.cell.2019.05.031 ↗
54. Luecken · Mol. Syst. Biol. · 2019 · 15 · e874610.15252/msb.20188746 ↗