journal article·2023

Injectable chondroitin sulfate-grafted self-antioxidant hydrogels ameliorate nucleus pulposus degeneration against overactive inflammation

Huitong Luo, Zetao Wang, Zhichao He, Zemin Ling, Hao Wang, Jiayi Zhu, Jingjun Nie, Dafu Chen, Qi Feng, Xiaodong Cao

Biomaterials Science· Volume 11· Issue 10
Publisher page

Abstract

Overactive inflammatory cascade accompanied by oxidative stress in the nucleus pulposus exacerbates intervertebral disc degeneration (IVDD). Hydrogels have been demonstrated to be promising in treating IVDD, yet they remain less efficacious in the case of anti-inflammation associated with antioxidation. In this study, we designed an injectable self-antioxidant hydrogel (HA/CS) with enhanced inflammation inhibitory performance for delivering chondroitin sulfate (CS) with well-documented anti-inflammatory property to treat IVDD. The hydrogel was rapidly formed via dynamic boronate ester bonding between furan/phenylboronic acid and furan/dopamine-modified hyaluronic acid (HA), and mechanically enhanced by Diels-Alder reaction-induced secondary crosslinking, partial dopamine groups of which contribute to grafting phenylboronic acid-modified CS (CS-PBA). This hydrogel exhibits favorable injectability, mechanical property, and pH-responsive delivery behavior. The dopamine moiety endows the hydrogel with efficient antioxidative property. By sustained delivery of CS, the HA/CS hydrogel is well competent to inhibit inflammatory cytokine expression and maintain anabolic/catabolic balance in an inflammation-simulated environment. Most importantly, the HA/CS hydrogel significantly ameliorates degeneration in a puncture-induced IVDD rat model. The self-antioxidant HA/CS hydrogel designed in this work may serve as a novel and promising therapeutic platform for IVDD.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Spine and Intervertebral Disc Pathology · Musculoskeletal pain and rehabilitation · Pain Mechanisms and Treatments

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510006, China

National Engineering Research Center for Tissue Restoration and Reconstruction (NERC-TRR), South China University of Technology, Guangzhou, 510006, China

Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, Department of Spinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China

School of Medicine, South China University of Technology, Guangzhou 510006, China

Laboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing, 100035, China

Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, China

Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou, 510641, China

Funding

National Key Research and Development Program of China · Beijing Municipal Health Commission · National Natural Science Foundation of China

References (63)

Show source-provided references
1. Balague · Lancet · 2012 · 379 · 48210.1016/S0140-6736(11)60610-7 ↗
2. Jensen · Pain · 2017 · 158 · 89110.1097/j.pain.0000000000000851 ↗
3. Adams · Spine · 2006 · 31 · 215110.1097/01.brs.0000231761.73859.2c ↗
4. Hicks · Spine · 2009 · 34 · 130110.1097/BRS.0b013e3181a18263 ↗
5. Vo · J. Orthop. Res. · 2016 · 34 · 128910.1002/jor.23195 ↗
6. Smith · Dis. Models Mech. · 2011 · 4 · 3110.1242/dmm.006403 ↗
7. Battie · J. Bone Jt. Surg., Am. Vol. · 2006 · 88A · 3
8. Vergroesen · Osteoarthritis Cartilage · 2015 · 23 · 105710.1016/j.joca.2015.03.028 ↗
9. Molinos · J. R. Soc., Interface · 2015 · 12 · 2015042910.1098/rsif.2015.0429 ↗
10. Risbud · Nat. Rev. Rheumatol. · 2014 · 10 · 4410.1038/nrrheum.2013.160 ↗
11. Cao · Oxid. Med. Cell. Longevity · 2022 · 2022 · 1052878
12. Cheng · Biomaterials · 2011 · 32 · 695310.1016/j.biomaterials.2011.03.065 ↗
13. Zheng · Theranostics · 2021 · 11 · 14710.7150/thno.47723 ↗
14. Li · Oxid. Med. Cell. Longevity · 2022 · 2022 · 1984742
15. Dai · Mater. Today Bio · 2023 · 18 · 10051210.1016/j.mtbio.2022.100512 ↗
16. Blaser · Trends Cell Biol. · 2016 · 26 · 24910.1016/j.tcb.2015.12.002 ↗
17. Yang · Apoptosis · 2015 · 20 · 34810.1007/s10495-015-1086-4 ↗
18. Harmon · Biomater. Sci. · 2020 · 8 · 121610.1039/C9BM01288E ↗
19. Xu · Adv. Funct. Mater. · 2020 · 200633310.1002/adfm.202006333 ↗
20. Bian · Nano Lett. · 2021 · 21 · 269010.1021/acs.nanolett.0c04713 ↗
21. Kamali · Theranostics · 2021 · 11 · 2710.7150/thno.48987 ↗
22. Silagi · Matrix Biol. · 2018 · 71–72 · 36810.1016/j.matbio.2018.02.025 ↗
23. Volpi · Inflammopharmacology · 2011 · 19 · 29910.1007/s10787-011-0098-0 ↗
24. Zhou · Acta Biomater. · 2017 · 63 · 6410.1016/j.actbio.2017.09.005 ↗
25. Wang · Nano Res. · 2023 · 16 · 2789
26. Reginster · Ann. Rheum. Dis. · 2017 · 76 · 153710.1136/annrheumdis-2016-210860 ↗
27. Sharma · Carbohydr. Polym. · 2022 · 286 · 11930510.1016/j.carbpol.2022.119305 ↗
28. du Souich · J. Cell. Mol. Med. · 2009 · 13 · 145110.1111/j.1582-4934.2009.00826.x ↗
29. Lim · Adv. Healthcare Mater. · 2022 · 11 · 210148310.1002/adhm.202101483 ↗
30. Lin · Mol. Ther.–Nucleic Acids · 2019 · 16 · 16210.1016/j.omtn.2019.02.012 ↗
31. Bai · Adv. Healthcare Mater. · 2020 · 9 · e190118610.1002/adhm.201901186 ↗
32. Xing · J. Nanobiotechnol. · 2021 · 19 · 26410.1186/s12951-021-00991-5 ↗
33. Chen · Bioact. Mater. · 2022 · 9 · 2910.1016/j.bioactmat.2021.08.003 ↗
34. He · Carbohydr. Polym. · 2023 · 299 · 12018010.1016/j.carbpol.2022.120180 ↗
35. Gan · Biomaterials · 2017 · 136 · 1210.1016/j.biomaterials.2017.05.017 ↗
36. Zhu · Biomater. Sci. · 2017 · 5 · 78410.1039/C7BM00001D ↗
37. Liu · Bioact. Mater. · 2021 · 6 · 14610.1016/j.bioactmat.2020.07.008 ↗
38. Farrugia · Biomater. Sci. · 2018 · 6 · 94710.1039/C7BM01158J ↗
39. Shi · Biofabrication · 2021 · 14 · 01410710.1088/1758-5090/ac42de ↗
40. Xu · ACS Appl. Mater. Interfaces · 2018 · 10 · 3949410.1021/acsami.8b15006 ↗
41. Li · ACS Nano · 2022 · 16 · 748610.1021/acsnano.1c10575 ↗
42. Zhang · Chem. Eng. J. · 2020 · 392 · 12377510.1016/j.cej.2019.123775 ↗
43. Zhao · ACS Appl. Mater. Interfaces · 2020 · 12 · 5123610.1021/acsami.0c14805 ↗
44. Yu · Polym. Chem. · 2020 · 11 · 316910.1039/D0PY00371A ↗
45. Shan · Polym. Chem. · 2017 · 8 · 299710.1039/C7PY00519A ↗
46. Schmitz · Acta Biomater. · 2020 · 114 · 110.1016/j.actbio.2020.08.001 ↗
47. Yu · ACS Appl. Mater. Interfaces · 2015 · 7 · 2402310.1021/acsami.5b06896 ↗
48. Yu · Polym. Chem. · 2014 · 5 · 511610.1039/C4PY00473F ↗
49. Gandini · Prog. Polym. Sci. · 2013 · 38 · 110.1016/j.progpolymsci.2012.04.002 ↗
50. Pfirrmann · Spine · 2001 · 26 · 187310.1097/00007632-200109010-00011 ↗
51. Han · Spine · 2008 · 33 · 192510.1097/BRS.0b013e31817c64a9 ↗
52. Zhang · ACS Appl. Mater. Interfaces · 2022 · 14 · 2640410.1021/acsami.2c04300 ↗
53. Chen · New J. Chem. · 2021 · 45 · 1071110.1039/D1NJ01228B ↗
54. Culbert · Biomater. Sci. · 2022 · 10 · 87410.1039/D1BM01589C ↗
55. Iatridis · J. Biomech. · 1997 · 30 · 100510.1016/S0021-9290(97)00069-9 ↗
56. Cloyd · Eur. Spine J. · 2007 · 16 · 189210.1007/s00586-007-0443-6 ↗
57. Bibby · Eur. Spine J. · 2004 · 13 · 69510.1007/s00586-003-0616-x ↗
58. Bibby · Spine · 2005 · 30 · 48710.1097/01.brs.0000154619.38122.47 ↗
59. Frapin · Adv. Drug Delivery Rev. · 2019 · 149–150 · 4910.1016/j.addr.2019.08.007 ↗
60. Le Maitre · Arthritis Res. Ther. · 2007 · 9 · R7710.1186/ar2275 ↗
61. Isa · Biomacromolecules · 2015 · 16 · 171410.1021/acs.biomac.5b00168 ↗
62. Sakai · Adv. Drug Delivery Rev. · 2015 · 84 · 15910.1016/j.addr.2014.06.009 ↗
63. Zhang · Cell Proliferation · 2021 · 54 · e1305710.1111/cpr.13057 ↗