Collagen immobilization of multi-layered BCP-ZrO 2 bone substitutes to enhance bone formation
Source abstract
Abstract
Abstract A porous microstructure of multi-layered BCP-ZrO 2 bone substitutes was fabricated using the sponge replica method in which the highly interconnected structure was immobilized with collagen via ethyl(dimethylaminopropyl)carbodiimide/ N -hydroxysuccinimide crosslinking. Their struts are combined with a three-layered BCP/BCP-ZrO 2 /ZrO 2 microstructure. Collagen fibers were firmly attached to the strut surface of the BCP-ZrO 2 scaffolds. With control of the three-layered microstructure and collagen immobilization, the compressive strength of the scaffolds increased significantly to 6.8 MPa compared to that of the monolithic BCP scaffolds (1.3 MPa). An in vitro study using MTT, confocal observation, and real-time polymer chain reaction analysis demonstrated that the proliferation and differentiation of the pre-osteoblast-like MC3T3-E1 cells was improved due to the collagen incorporation. Remarkable enhancement of bone regeneration was observed without any immunological reaction in the femurs of rabbits during 1 and 5 months of implantation. Furthermore, the interfaces between new bone and the scaffold struts bonded directly without any gaps.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Orthopaedic implants and arthroplasty · Dental Implant Techniques and Outcomes
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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