Comparison of in situ and ex situ bioconjugation of Au nanoparticles generated by laser ablation
Source abstract
Abstract
Abstract Au nanoparticles were generated by laser ablation in PBS buffer and conjugated to immunoglobulin E (IgE) during ablation ( in situ ) and after ablation ( ex situ ). Exposure for 5 min to 532 nm pulses with a duration of 150 ps, an energy of 8 mJ and a repetition rate of 10 Hz yielded nanoparticles with a mean diameter of about 4 nm for in situ conjugation and of about 5 nm for ex situ conjugation. ELISA analysis showed that the conjugation efficiency was comparable for in situ and ex situ fabrication. ELISA for cytokine (IL-6) production by IgE-activated mast cells showed that the Au–IgE conjugates induced a response which coincided within error for conjugates prepared in situ and ex situ.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Laser-Ablation Synthesis of Nanoparticles · Nonlinear Optical Materials Studies · Gold and Silver Nanoparticles Synthesis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (14)
Show source-provided references
1. Laser ablation synthesis in solution and size manipulation of noble metal nanoparticles10.1039/b900654k ↗
2. Laser ablation in liquids: applications in the synthesis of nanocrystals10.1016/j.pmatsci.2006.10.016 ↗
3. Ultrafast laser based green synthesis of non-toxic nanoparticles in aqueous solutions10.1007/s00339-008-4773-y ↗
4. Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers10.1186/1477-3155-8-21 ↗
5. Growth of gold clusters into nanoparticles in a solution following laser-induced fragmentation10.1021/jp020786i ↗
6. Delay time concentration effects during bioconjugation of nanosecond laser-generated nanoparticles in a liquid flow10.1021/jp1093405 ↗
7. Surface chemistry of gold nanoparticles produced by laser ablation in aqueous media10.1021/jp047134+ ↗
8. Selective degradation of proteins by laser irradiation onto gold nanoparticles in solution10.1021/jp809438d ↗
9. Labeling of colloidal gold with IgE: a quantitative study using monoclonal IgE anti-β-lactoglobulin and evaluation of the biological activity of the gold complex with RBL-1 cells
10. In vivo murine CD23 destabilization enhances CD23 shedding and IgE synthesis10.1016/j.cellimm.2007.01.004 ↗
11. Novel mechanism for fc RI-mediated signal transducer and activator of transcription 5 (STAT5) tyrosine phosphorylation and the selective influence of STAT5B over mast cell cytokine production10.1074/jbc.M111.311142 ↗
12. Magnetic nanoparticles of iron carbide, iron oxide, iron@iron oxide, and metal iron synthesized by laser ablation in organic solvents10.1021/jp109371m ↗
13. Controlled size manipulation of free gold nanoparticles by laser irradiation and their facile bioconjugation10.1039/b709621f ↗
14. Size evaluation of gold nanoparticles by UV–Vis spectroscopy10.1021/jp8082425 ↗