journal article·2008-06

Grazing-incidence small-angle X-ray scattering from alkaline phosphatase immobilized in atmospheric plasmapolymer coatings

M.G. Ortore, R. Sinibaldi, P. Heyse, S. Paulussen, S. Bernstorff, B. Sels, P. Mariani, F. Rustichelli, F. Spinozzi

Applied Surface Science· Volume 254· Issue 17
Publisher page

Abstract

Grazing-incidence small-angle X-ray scattering (GISAXS) has been used to study proteins embedded in thin polymer films obtained by a new cold, atmospheric-pressure plasma technique. In order to test the efficiency of the technology, four samples of alkaline phosphatase incorporated in organic polymer coatings in different plasma conditions have been investigated. Data have been analysed in the framework of the distorted-wave Born approximation (DWBA), by using a new method for the simultaneous fitting of the two-dimensional diffuse scattering from each sample. As a result, protein film concentration and aggregation state as well as a set of parameters describing the polymer coatings have been obtained.
Source: openaire · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Ion-surface interactions and analysis · Theoretical and Computational Physics · Enzyme Structure and Function

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

References (25)

Show source-provided references
1. Electrochemical protein chip with arrayed immunosensors with antibodies immobilized in a plasma-polymerized film10.1021/ac0257391 ↗
2. Structure and biosensor characteristics of complex between glucose oxidase and plasma-polymerized nanothin film10.1016/j.bios.2006.02.017 ↗
3. Dielectric barrier discharge at atmospheric pressure as a tool to deposit versatile organic coatings at moderate power input10.1002/ppap.200600087 ↗
4. Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi10.1128/AEM.67.10.4504-4511.2001 ↗
5. Real-time monitoring of growing nanoparticles10.1126/science.1082146 ↗
6. X-ray and neutron scattering from rough surfaces10.1103/PhysRevB.38.2297 ↗
7. Influence of the roughness profile on the specular reflectivity of X-rays and neutrons10.1103/PhysRevB.49.5817 ↗
8. Non specular X-ray reflection from rough multilayers10.1103/PhysRevB.49.10668 ↗
9. Grazing-incidence small-angle X-ray scattering from free-standing nanostructures10.1063/1.371724 ↗
10. Isgisaxs: a program for grazing-incidence small-angle X-ray scattering analysis of supported islands10.1107/S0021889802006088 ↗
11. In situ gisaxs study of the growth of Pd on MgO(0 0 1)10.1016/j.apsusc.2004.05.235 ↗
12. Grazing-incidence small-angle X-ray scattering from thin polymer films with lamellar structures - the scattering cross section in the distorted-wave Born approximation10.1107/S0021889806012337 ↗
13. High pressure small-angle neutron scattering study of the aggregation state of β-lactoglobulin in water and water/ethylene glycol solutions
14. Application of atmospheric pressure dielectric barrier discharges in deposition, cleaning and activation10.1016/S0257-8972(01)01140-9 ↗
15. Physical and chemical properties of hybrid barrier coatings obtained in an atmospheric pressure dielectric barrier discharge10.1088/0022-3727/38/4/008 ↗
16. R. Lazzari, 2006. http://www.insp.upmc.fr/axe2/Oxydes/IsGISAXS/isgisaxs.htm.
17. Effects of grazing incidence conditions on the X-ray diffuse scattering from self-assembled nanoscale islands10.1103/PhysRevB.71.115324 ↗
18. Ultrathin diblock copolymer/titanium laminates—a tool for nanolithography10.1002/(SICI)1521-4095(199808)10:11<849::AID-ADMA849>3.0.CO;2-5 ↗
19. X-ray reflectivity and diffuse scattering
20. X-ray diffraction from Si/Ge layers: diffuse scattering in the region of total external reflection10.1103/PhysRevB.51.2311 ↗
21. Surface studies of solids by total reflection of X-rays10.1103/PhysRev.95.359 ↗
22. F. Spinozzi, 2008. http://alisf1.univpm.it/biophysics/software.htm
23. Interaction of proteins in solution from small angle scattering: a perturbative approach10.1016/S0006-3495(02)75563-X ↗
24. Surface and interface topography of amorphous SiO2crystalline Si(100) studied by X-ray diffraction10.1088/0953-8984/2/45/003 ↗
25. Diffuse X-ray scattering study of interfacial structure of self-assembled conjugated polymers10.1103/PhysRevB.66.161201 ↗