Spatial modulation of biomolecules immobilization by fabrication of hierarchically structured PEG-derived brush micropatterns: An versatile cellular microarray platform
Source abstract
Abstract
Abstract Microarray technology holds enormous promise in the development of various fields ranging from tissue engineering, regenerative medicine to high-throughput screening. Here, based on the digital micromirror device (DMD)-based spatiotemporal regulation of surface-initiated photoinduced atom transfer radical polymerization (Photo-ATRP) process, a flexible and versatile methodology was developed to fabricate a hierarchical microarray structure constituted with a homogeneous hyperbranched polyethylene glycol (PEG)-derived brush layer as anti-fouling background and an extension layer of square-grid poly(2-(2-azido-2-methyl-1-oxopropoxy) ethyl methacrylate) (PAMEMA) brushes micropatterns on the silicon substrate. The terminal azido groups on the side chains of PAMEMA brushes provide abundant reactive sites to realize the covalent immobilization of target biomolecules, including RGD peptide, fibronectin, BSA and streptavidin. The TOF-SIMS and fluorescence characterizations demonstrated the feasibility and efficiency of spatially modulating the density of surface-bound biomolecules through regulating the 3D architecture parameters of the PAMEMA brush micropatterns and consequentially the azido chemical functionality. Moreover, culture experiments of human bone-derived marrow stromal cells (BMSCs) and mouse L929 cells were conducted on the obtained hierarchical microarray structure in a high-throughput manner. The presented hierarchical microarray structure holds excellent potential as a high-throughput screening platform, allowing for the parallel assessment of cell-surface interactions.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanofabrication and Lithography Techniques · Advanced Biosensing Techniques and Applications · Monoclonal and Polyclonal Antibodies Research
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References (45)
Show source-provided references
1. T.G. Fernandes, M.M. Diogo, D.S. Clark, J.S. Dordick, J.M. Cabral, High-throughput cellular microarray platforms: applications in drug discovery, toxicology and stem cell research, Trends Biotechnol. 27 (2009) 342-349.https://doi.org/10.1016/j.tibtech.2009.02.009.10.1016/j.tibtech.2009.02.009 ↗
2. A.D. Celiz H.C. Harrington A.L. Hook High throughput assessment and chemometric analysis of the interaction of epithelial and fibroblast cells with a polymer library Appl. Surf. Sci. 313 (2014) 926–935.https: //doi.org/10.1016/j.apsusc.2014.06.111.10.1016/j.apsusc.2014.06.111 ↗
3. O.I. Berthuy, S.K. Muldur, F. Rossi, P. Colpo, L.J. Blum, C.A. Marquette, Multiplex cell microarrays for high-throughput screening, Lab Chip 16 (2016) 4248-4262. Doi: 10.1039/c6lc00831c.10.1039/C6LC00831C ↗
4. W.J. Brittain T. Brandsetter O. Prucker J. Rühe The Surface Science of Microarray Generation-A Critical Inventory ACS Appl. Mater. Interfaces 11 2019 39397–39409.https: //doi.org/10.1021/acsami.9b06838.10.1021/acsami.9b06838 ↗
5. Surface Engineering for Long-Term Culturing of Mesenchymal Stem Cell Microarrays10.1021/bm400531n ↗
6. J.-W. Jang, J.M. Collins, S. Nettikadan, User-Friendly Universal and Durable Subcellular-Scaled Template for Protein Binding: Application to Single-Cell Patterning, Adv. Funct. Mater. 23 (2013) 5840-5845.https://doi.org/10.1002/adfm.201301088.10.1002/adfm.201301088 ↗
7. Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale10.1016/j.biomaterials.2010.02.066 ↗
8. Z. Tong, G. Rajeev, K. Guo, A. Ivask, S. McCormick, E. Lombi, C. Priest, N.H. Voelcker, Microfluidic Cell Microarray Platform for High Throughput Analysis of Particle-Cell Interactions, Anal. Chem. 90 (2018) 4338-4347.https://doi.org/10.1021/acs.analchem.7b03079.10.1021/acs.analchem.7b03079 ↗
9. T.-Y. Chang, M. Huang, A.A. Yanik, H.-Y. Tsai, P. Shi, S. Aksu, M.F. Yanik, H. Altug, Large-scale plasmonic microarrays for label-free high-throughput screening, Lab Chip 11 (2011) 3596-3602.https://doi.org/10.1039/c1lc20475k.10.1039/c1lc20475k ↗
10. J.B. You, Y. Wang, C. McCallum, F. Tarlan, T. Hannam, A. Lagunov, K. Jarvi, D. Sinton, Live sperm trap microarray for high throughput imaging and analysis, Lab Chip 19 (2019) 815-824.https://doi.org/10.1039/c8lc01204k.10.1039/C8LC01204K ↗
11. Z. Lei, H.D. Chen, H. Zhang, Y.Q. Wang, X.Y. Meng, Z.X. Wang, Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment, ACS Appl. Mater. Interfaces 9 (2017) 44241-44250.https://doi.org/10.1021/acsami.7b15445.10.1021/acsami.7b15445 ↗
12. M.H. Jian, M. Su, J.X. Gao, Z.X. Wang, Peptide microarray-based fluorescence assay for quantitatively monitoring the tumor-associated matrix metalloproteinase-2 activity, Sensors Actuators B: Chem. 304 (2020) 127320.https://doi.org/10.1016/j.snb.2019.127320.10.1016/j.snb.2019.127320 ↗
13. S.Y. Li, X. Wang, B. Cao, K. Ye, Z.H. Li, J.D. Ding, Effects of Nanoscale Spatial Arrangement of Arginine-Glycine-Aspartate Peptides on Dedifferentiation of Chondrocytes, Nano Lett. 15 (2015) 7755-7765.https://doi.org/10.1021/acs.nanolett.5b04043.10.1021/acs.nanolett.5b04043 ↗
14. X. Wang, S.Y. Li, C. Yan, P. Liu, J.D. Ding, Fabrication of RGD Micro/Nanopattern and Corresponding Study of Stem Cell Differentiation, Nano Lett. 15 (2015) 1457-1467.https://doi.org/10.1021/nl5049862.10.1021/nl5049862 ↗
15. R. Coyle, J. Jia, Y. Mei, Polymer microarray technology for stem cell engineering, Acta Biomater. 34 (2016) 60-72.https://doi.org/10.1016/j.actbio.2015.10.030.10.1016/j.actbio.2015.10.030 ↗
16. I. Pereiro, J.F. Cors, S. Pane, G.V. Kaigala, Underpinning transport phenomena for the patterning of biomolecules, Chem. Soc. Rev. 48 (2019) 1236-1254.https://doi.org/10.1039/C8CS00852C.10.1039/C8CS00852C ↗
17. High-throughput approaches for screening and analysis of cell behaviors10.1016/j.biomaterials.2017.06.022 ↗
18. S. Lamping, C. Buten, B.J. Ravoo, Functionalization and Patterning of Self-Assembled Monolayers and Polymer Brushes Using Microcontact Chemistry, Acc. Chem. Res. 52 (2019) 1336-1346.https://doi.org/10.1021/acs.accounts.9b00041.10.1021/acs.accounts.9b00041 ↗
19. K. Gajos, A. Budkowski, P. Petrou, K. Awsiuk, K. Misiakos, I. Raptis, S. Kakabakos, Spatially selective biomolecules immobilization on silicon nitride waveguides through contact printing onto plasma treated photolithographic micropattern: Step-by-step analysis with TOF-SIMS chemical imaging, Appl. Surf. Sci. 506 (2020) 145002. Doi: 10.1016/j.apsusc.2019.145002.10.1016/j.apsusc.2019.145002 ↗
20. Y.S. Park H.G. Son D.H. Kim H.G. Oh D.S. Lee M.H. Kim K.M. Lim K.S. Song Microarray of neuroblastoma cells on the selectively functionalized nanocrystalline diamond thin film surface Appl. Surf. Sci. 361 2016 269–276.https: //doi.org/0.1016/j.apsusc.2015.11.012.10.1016/j.apsusc.2015.11.012 ↗
21. V. Romanov, S.N. Davidoff, A.R. Miles, D.W. Grainger, B.K. Gale, B.D. Brooks, A critical comparison of protein microarray, Analyst 139 (2014) 1303-1326.https://doi.org/10.1039/c3an01577g.10.1039/C3AN01577G ↗
22. S. Sekula-Neuner, J. Maier, E. Oppong, A.C.B. Cato, M. Hirtz, H. Fuchs, Allergen Arrays for Antibody Screening and Immune Cell Activation Profiling Generated by Parallel Lipid Dip-Pen Nanolithography, Small 8 (2012) 585-591.https://doi.org/10.1002/smll.201101694.10.1002/smll.201101694 ↗
23. A. Vaish, M.J. Shuster, S. Cheunkar, P.S. Weiss, A.M. Andrews, Tuning Stamp Surface Energy for Soft Lithography of Polar Molecules to Fabricate Bioactive Small-Molecule Microarrays, Small 7 (2011) 1471-1479.https://doi.org/10.1002/smll.201100094.10.1002/smll.201100094 ↗
24. Development of Sphere-Polymer Brush Hierarchical Nanostructure Substrates for Fabricating Microarrays with High Performance10.1021/acsami.7b09505 ↗
25. Development of super-dense transfected cell microarrays generated by piezoelectric inkjet printing10.1039/C2LC40709D ↗
26. F. Loeffler, C. Schirwitz, J. Wagner, K. Koenig, F. Maerkle, G. Torralba, M. Hausmann, F.R. Bischoff, A. Nesterov-Mueller, F. Breitling, Biomolecule Arrays Using Functional Combinatorial Particle Patterning on Microchips, Adv. Funct. Mater. 22 (2012) 2503–2508.https://doi.org/10.1002/adfm.201103103.10.1002/adfm.201103103 ↗
27. L. An G.T. Wang Y. Han T.C. Li P. Jin S.Q. Liu Electrochemical biosensor for cancer cell detection based on a surface 3D micro-array Lab Chip 18 2018 335–342.https: //doi.org/10.1039/c7lc01117b.10.1039/C7LC01117B ↗
28. Directing cell migration using micropatterned and dynamically adhesive polymer brushes10.1016/j.actbio.2014.01.029 ↗
29. Biomimetic Polymer Brushes Containing Tethered Acetylcholine Analogs for Protein and Hippocampal Neuronal Cell Patterning10.1021/bm301785b ↗
30. Direct Write Protein Patterns for Multiplexed Cytokine Detection from Live Cells Using Electron Beam Lithography10.1021/acsnano.5b05781 ↗
31. E. Ueda, W.Q. Feng, P.A. Levkin, Superhydrophilic-Superhydrophobic Patterned Surfaces as High-Density Cell Microarrays: Optimization of Reverse Transfection, Adv. Healthcare Mater. 5 (2016) 2646-2654.https://doi.org/10.1002/adhm.201600518.10.1002/adhm.201600518 ↗
32. J. Lietard, D. Ameur, M.J. Damha, M.M. Somoza, High-Density RNA Microarrays Synthesized In Situ by Photolithography, Angew.Chem. Int.Ed. 57 (2018) 15257-15261.https://doi.org/10.1002/ange.201806895.10.1002/anie.201806895 ↗
33. A.K. Gaharwar A. Arpanaei T.L. Andresen A. Dolatshahi-Pirouz, 3D Biomaterial Microarrays for Regenerative Medicine: Current State-of-the-Art, Emerging Directions and Future Trends, Adv. Mater. 28 2016 771–781.https: //doi.org/10.1002/adma.201503918.10.1002/adma.201503918 ↗
34. G.C. Li S.J. Li L.L. Zhang S.Y. Chen Z.D. Sun S.Q. Li L.Z. Zhang Y.M. Yang Construction of Biofunctionalized Anisotropic Hydrogel Micropatterns and Their Effect on Schwann Cell Behavior in Peripheral Nerve Regeneration ACS Appl. Mater. Interfaces 11 2019 37397–37410.https: //doi.org/10.1021/acsami.9b08510.10.1021/acsami.9b08510 ↗
35. U. Bog, A. de Los Santos Pereira, S.L. Mueller, S. Havenridge, V. Parrillo, M. Bruns, A.E. Holmes, C. Rodriguez-Emmenegger, H. Fuchs, M. Hirtz, Clickable Antifouling Polymer Brushes for Polymer Pen Lithography, ACS Appl. Mater. Interfaces 9 (2017) 12109-12117.https://doi.org/10.1021/acsami.7b01184.10.1021/acsami.7b01184 ↗
36. H.L. Zhao J. Sha X.F. Wang Y.C. Jiang T. Chen T. Wu X. Chen H.J. Ji Y. Gao L.S. Xie Y.L. Ma Spatiotemporal control of polymer brush formation through photoinduced radical polymerization regulated by DMD light modulation Lab Chip 2651–2662.https 19 (2019) //doi.org/10.1039/C9LC00419J.10.1039/C9LC00419J ↗
37. A. Waldbaur, B. Waterkotte, K. Schmitz, B.E. Rapp, Maskless Projection Lithography for the Fast and Flexible Generation of Grayscale Protein Patterns, Small 8 (2012) 1570-1578.https://doi.org/10.1002/smll.201102163.10.1002/smll.201102163 ↗
38. A.P. Zhang X. Qu P. Soman K.C. Hribar J.W. Lee S.C. Chen S. He Rapid fabrication of complex 3D extracellular microenvironments by dynamic optical projection stereolithography Adv. Mater. 24 2012 4266–4270.https: //doi.org/10.1002/adma.201202024.10.1002/adma.201202024 ↗
39. DMD-based LED-illumination Super-resolution and optical sectioning microscopy10.1038/srep01116 ↗
40. X. Huang, J. He, Y. Hao, M. Ye, Q. Zhang, P. Ni, J. Liu, Synthesis of PEGylated brush-type copolymers for a plurality of plug-and-play functions, RSC Advances 5 (2015) 50019-50023.https://doi.org/10.1039/c5ra06484h.10.1039/C5RA06484H ↗
41. X.C. Zhou, X.Q. Liu, Z. Xie, Z.J. Zheng, 3D-patterned polymer brush surfaces, Nanoscale 3 (2011) 4929.https://doi.org/10.1039/c1nr11238d.10.1039/c1nr11238d ↗
42. Z. Xie, C. Chen, X. Zhou, T. Gao, D. Liu, Q. Miao, Z. Zheng, Massively parallel patterning of complex 2D and 3D functional polymer brushes by polymer pen lithography, ACS Appl. Mater. Interfaces 6 (2014) 11955-11964.https://doi.org/10.1021/am405555e.10.1021/am405555e ↗
43. Sean Lowe, N.M. O'Brien-Simpson, L.A. Connal, Antibiofouling polymer interfaces: Poly(ethylene glycol) and other promising candidates, Polym. Chem. 6 (2015) 198-212.https://doi.org/10.1039/C4PY01356E.10.1039/C4PY01356E ↗
44. V. Parrillo A. de Los Santos T. Pereira C. Rodriguez-Emmenegger Riedel Catalyst-free “click” functionalization of polymer brushes preserves antifouling properties enabling detection in blood plasma Anal. Chim. Acta 971 2017 78–87.https: //doi.org/10.1016/j.aca.2017.03.007.10.1016/j.aca.2017.03.007 ↗
45. Fabrication of Two-Component, Brush-on-Brush Topographical Microstructures by Combination of Atom-Transfer Radical Polymerization with Polymer End-Functionalization and Photopatterning10.1021/acs.langmuir.5b01067 ↗