journal article·2016-03

Probing stem cell differentiation using atomic force microscopy

Xiaobin Liang, Xuetao Shi, Serge Ostrovidov, Hongkai Wu, Ken Nakajima

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Abstract

Abstract A real-time method using atomic force microscopy (AFM) was developed to probe stem cell differentiation by measuring the mechanical properties of cells and the extracellular matrix (ECM). The mechanical properties of stem cells and their ECMs can be used to clearly distinguish specific stem cell-differentiated lineages. It is clear that AFM is a facile and useful tool for monitoring the differentiation of stem cells in a non-invasive manner.
Source: openaire · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Cellular Mechanics and Interactions · Force Microscopy Techniques and Applications · Microfluidic and Bio-sensing Technologies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

References (42)

Show source-provided references
1. Mechanical memory and dosing influence stem cell fate10.1038/nmat3889 ↗
2. Cellular mechanical properties reflect the differentiation potential of adipose-derived mesenchymal stem cells10.1073/pnas.1120349109 ↗
3. Quantifying cell-generated mechanical forces within living embryonic tissues10.1038/nmeth.2761 ↗
4. Mechanical regulation of cell function with geometrically modulated elastomeric substrates10.1038/nmeth.1487 ↗
5. Mechanisms of cytoskeleton-mediated mechanical signal transmission in cells10.4161/cib.21633 ↗
6. Mechanical signal influence on mesenchymal stem cell fate is enhanced by incorporation of refractory periods into the loading regimen10.1016/j.jbiomech.2010.11.022 ↗
7. A mechanical model of cell segregation driven by differential adhesion10.1371/journal.pone.0043226 ↗
8. Mechanical control of morphogenesis by Fat/Dachsous/Four-jointed planar cell polarity pathway10.1126/science.1221071 ↗
9. The fundamental role of mechanical properties in the progression of cancer disease and inflammation10.1088/0034-4885/77/7/076602 ↗
10. Comparison of mechanical properties of normal and malignant thyroid cells10.1016/j.micron.2012.03.023 ↗
11. Stretchable and micropatterned membrane for osteogenic differentation of stem cells10.1021/am5029236 ↗
12. AFM indentation study of breast cancer cells10.1016/j.bbrc.2008.07.078 ↗
13. Cancer cell detection in tissue sections using AFM10.1016/j.abb.2011.12.013 ↗
14. Stem cell plasticity
15. Elasticity mapping of living fibroblasts by AFM and immunofluorescence observation of the cytoskeleton10.1016/S0304-3991(99)00157-6 ↗
16. Mechanical derivation of functional myotubes from adipose-derived stem cells10.1016/j.biomaterials.2011.12.004 ↗
17. Chip-based comparison of the osteogenesis of human bone marrow-and adipose tissue-derived mesenchymal stem cells under mechanical stimulation10.1371/journal.pone.0046689 ↗
18. Organogenesis from stem cells in planta: multiple feedback loops integrating molecular and mechanical signals10.1007/s00018-011-0732-4 ↗
19. Nanotopography-induced changes in focal adhesions, cytoskeletal organization, and mechanical properties of human mesenchymal stem cells10.1016/j.biomaterials.2009.10.037 ↗
20. Mesenchymal stem cells, cancer challenges and new directions10.1016/j.ejca.2014.02.011 ↗
21. AFM-based analysis of human metastatic cancer cells10.1088/0957-4484/19/38/384003 ↗
22. Method for quantitative measurements of the elastic modulus of biological cells in AFM indentation experiments10.1016/j.ymeth.2013.03.037 ↗
23. Elastic moduli of living epithelial pancreatic cancer cells and their skeletonized keratin intermediate filament network10.1116/1.3601755 ↗
24. Cellular mechanoadaptation to substrate mechanical properties: contributions of substrate stiffness and thickness to cell stiffness measurements using AFM10.1039/c3sm51786a ↗
25. AFM-based dual nano-mechanical phenotypes for cancer metastasis10.1007/s10867-014-9353-0 ↗
26. High-speed AFM for scanning the architecture of living cells10.1039/c3nr01464a ↗
27. Quantitative study of the elastic modulus of loosely attached cells in AFM indentation experiments10.1016/j.bpj.2013.04.019 ↗
28. Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells10.1038/nmeth.1320 ↗
29. The number distribution of complex shear modulus of single cells measured by atomic force microscopy10.1016/j.ultramic.2009.03.008 ↗
30. Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy10.1063/1.3672225 ↗
31. Nanomechanical analysis of cells from cancer patients10.1038/nnano.2007.388 ↗
32. Measurement of elastic properties of prostate cancer cells using AFM10.1039/b803355b ↗
33. Xia · ACS Nano · 2014 · 8 · 687310.1021/nn5015837 ↗
34. Zhang · Chem. Soc. Rev. · 2014 · 43 · 741210.1039/C4CS00176A ↗
35. Characterization of surface viscoelasticity and energy dissipation in a polymer film by atomic force microscopy10.1021/ma201148f ↗
36. Visualization of nanomechanical mapping on polymer nanocomposites by AFM force measurement10.1016/j.polymer.2010.03.052 ↗
37. Uber die Beruhrung fester elastischer Korper10.1515/crll.1882.92.156 ↗
38. Dielectrophoretically aligned carbon nanotubes to control electrical and mechanical properties of hydrogels to fabricate contractile muscle myofibers10.1002/adma.201301300 ↗
39. Periosteum-mimetic structures made from freestanding microgrooved nanosheets10.1002/adma.201305804 ↗
40. Gradient-regulated hydrogel for interface tissue engineering: steering simultaneous osteo/chondrogenesis of stem cells on a chip10.1002/adhm.201200333 ↗
41. Quantitative dynamics of adipose cells10.4161/adip.19705 ↗
42. CD271/p75(NTR) inhibits the differentiation of mesenchymal stem cells into osteogenic, adipogenic, chondrogenic, and myogenic lineages10.1089/scd.2010.0299 ↗