Three-dimensional biomolecule patterning
Source abstract
Abstract
A new method is demonstrated, where three-dimensional protein structures are made by employing multi-photon polymerization and photobiotin photolysis. The technique enables the construction of arbitrary two- and three-dimensional shapes with submicron resolution. The integrity of the immobilized biotin is confirmed by derivatization with fluorescently labeled streptavidin. Fluorescence microscopy is used in order to visualize the distribution of fluorescent streptavidin on the 3D structure.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanofabrication and Lithography Techniques · Nonlinear Optical Materials Studies · Advanced Fluorescence Microscopy Techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (14)
Show source-provided references
1. Fodor · Science · 1991 · 251 · 76710.1126/science.1990438 ↗
2. Wilde · Analyst · 2001 · 126 · 19510.1039/b008475l ↗
3. Rozsnyai · Angew. Chem. Int. Ed. · 1992 · 31 · 75910.1002/anie.199207591 ↗
4. Holden · J. Am. Chem. Soc. · 2003 · 125 · 807410.1021/ja035390e ↗
5. Choi · Anal. Biochem. · 2005 · 347 · 6010.1016/j.ab.2005.08.015 ↗
6. Balakirev · Anal. Chem. · 2005 · 77 · 547410.1021/ac0504619 ↗
7. Smith · Top. Curr. Chem. · 2006 · 261 · 6310.1007/128_017 ↗
8. Farsari · Opt. Lett. · 2005 · 30 · 318010.1364/OL.30.003180 ↗
9. Maruo · Opt. Lett. · 1997 · 22 · 13210.1364/OL.22.000132 ↗
10. Deubel · Nat. Mater. · 2004 · 3 · 44410.1038/nmat1155 ↗
11. Sun · Appl. Phys. Lett. · 1999 · 74 · 78610.1063/1.123367 ↗
12. Serbin · Opt. Express · 2004 · 12 · 522110.1364/OPEX.12.005221 ↗
13. Galajda · Appl. Phys. Lett. · 2001 · 78 · 24910.1063/1.1339258 ↗
14. Sun · Appl. Phys. Lett. · 2001 · 79 · 317310.1063/1.1418024 ↗