Bio-conjugation of curcumin with self-assembled casein nanostructure via surface loading enhances its bioactivity: An efficient therapeutic system
Source abstract
Abstract
Abstract In this study, self-assembled nanostructure of casein (NSCS) of spherical shape was prepared using an optimized desolvation method with a mean size of 45 nm. Curcumin (Cur), a potent natural antioxidant, an anticancer and poorly soluble agent in aqueous solution, was conjugated to protein nanostructure (NSCS-Cur) using CDI chemical activation method. We further, evaluated the physicochemical stability as well as the biological activity of NSCS-Cur in vitro. When the antioxidant activity (free radical scavenging activity) and cellular antioxidant activity (CAA) assay of nano-formulated curcumin (NSCS-Cur) was evaluated, it revealed that antioxidant activity of NSCS-Cur was higher than that of free Cur in both. Furthermore, we showed that the cytotoxicity of nano-formulated curcumin (NSCS-Cur) was found higher in four different cancer cells (breast cancer; MCF-7 and MDAMB231, cervical cancer; HeLa, osteosarcoma; MG 63) than that of free curcumin. Moreover, the conjugation with folic acid with NSCS-Cur (NSCS-Cur–FA) demonstrated enhanced cytotoxicity in cancer cells than un-conjugated one. When we studied the storage stability and bioactivity, NSCS-Cur formulation showed significant stability (up to 30 days) with ignorable loss in its bioactivity. Hence, we concluded from our findings that curcumin loaded protein nanostructure (NSCS-Cur) might be used to achieve higher stability, enhanced dispersibility in suspension as well as improved bioactivity of curcumin which also can be used in the pharmaceutical application.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Proteins in Food Systems · Curcumin's Biomedical Applications · Protein Hydrolysis and Bioactive Peptides
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (55)
Show source-provided references
1. Isoflavonoids and lignans have different potentials to modulate oxidative genetic damage in human colon cells10.1093/carcin/21.6.1247 ↗
2. The role of nutraceuticals in pancreatic cancer prevention and therapy: targeting cellular signaling, miRNAs and epigenome10.1097/MPA.0000000000000257 ↗
3. Dietary phytochemicals and cancer prevention: Nrf2 signaling, epigenetics, and cell death mechanisms in blocking cancer initiation and progression10.1016/j.pharmthera.2012.09.008 ↗
4. Nutrients from fruit and vegetable consumption reduce the risk of pancreatic cancer10.1007/s12029-012-9441-y ↗
5. Antioxidant intake and pancreatic cancer risk10.1002/cncr.27936 ↗
6. Perspectives for cancer prevention with natural compounds10.1200/JCO.2008.20.6235 ↗
7. Curcumin nanoformulations: a review of pharmaceutical properties and preclinical studies and clinical data related to cancer treatment10.1016/j.biomaterials.2013.12.090 ↗
8. Bioavailability of curcumin: problems and promises10.1021/mp700113r ↗
9. Role of curcumin in health and disease10.1080/13813450802033958 ↗
10. Chemopreventive and therapeutic effects of curcumin10.1016/j.canlet.2004.09.041 ↗
11. In vitro testing of curcumin based composites coatings as antitumoral systems against osteosarcoma cells10.1016/j.apsusc.2017.07.090 ↗
12. Stability of curcumin in buffer solutions and characterization of its degradation products10.1016/S0731-7085(96)02024-9 ↗
13. Encapsulation of curcumin in cationic micelles suppresses alkaline hydrolysis10.1021/la800780w ↗
14. Biocompatible, biodegradable and thermo-sensitive chitosan-g-poly (N-isopropylacrylamide) nanocarrier for curcumin drug delivery10.1016/j.ijbiomac.2011.04.008 ↗
15. Electrospinning process: versatile preparation method for biodegradable and natural polymers and biocomposite systems applied in tissue engineering and drug delivery10.1016/j.apsusc.2014.01.098 ↗
16. Nanohybrid organic–inorganic chitosan/dopamine/TiO2 composites with controlled drug-delivery properties10.1016/j.apsusc.2015.03.028 ↗
17. Synthesis and characterization of polymer-coated manganese ferrite nanoparticles as controlled drug delivery10.1016/j.apsusc.2017.09.096 ↗
18. Study on drug release of and biological response to UHMWPE wear debris carrying estradiol10.1016/j.apsusc.2012.04.064 ↗
19. Preparation and evaluation of BSA-based hydrosol nanoparticles cross-linked with genipin for oral administration of poorly water-soluble curcumin10.1016/j.ijbiomac.2017.06.083 ↗
20. Casein-based formulations as promising controlled release drug delivery systems10.1016/j.jconrel.2011.02.010 ↗
21. Protein-based nanocarriers as promising drug and gene delivery systems10.1016/j.jconrel.2012.04.036 ↗
22. Gelatin microspheres crosslinked with D,L-glyceraldehyde as a potential drug delivery system: preparation, characterisation, in vitro and in vivo studies10.1016/S0378-5173(00)00681-5 ↗
23. Enhanced oral bioavailability of calcium using bovine serum albumin microspheres10.3109/10717544.2012.704094 ↗
24. Preparation, characterization and in vivo distribution of terbutaline sulfate loaded albumin microspheres10.1016/S0168-3659(02)00141-4 ↗
25. Preparation and evaluation of thiol-modified gelatin nanoparticles for intracellular DNA delivery in response to glutathione10.1021/bc050146t ↗
26. Milk proteins as vehicles for bioactives10.1016/j.cocis.2009.11.002 ↗
27. Beta-casein nanovehicles for oral delivery of chemotherapeutic drugs10.1016/j.nano.2009.06.006 ↗
28. Acidic solution properties of β-casein-graft-dextran copolymer prepared through Maillard reaction10.1016/j.jcis.2006.04.048 ↗
29. New views of protein structure: applications to the caseins: protein structure and functionality
30. Fabrication and delivery properties of soy Kunitz trypsin inhibitor nanoparticles10.1039/C6RA19886D ↗
31. pH-driven encapsulation of curcumin in self-assembled casein nanoparticles for enhanced dispersibility and bioactivity10.1039/C4SM00239C ↗
32. Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements10.1021/jf0715166 ↗
33. Folate-mediated targeted and intracellular delivery of paclitaxel using a novel deoxycholic acid-O-carboxymethylated chitosan–folic acid micelles
34. Self-assembled cationic peptide nanoparticles as an efficient antimicrobial agent10.1038/nnano.2009.153 ↗
35. Kouchakzadeh · 2015 · 121
36. Beta casein-micelle as a nano vehicle for solubility enhancement of curcumin; food industry application10.1016/j.lwt.2011.05.023 ↗
37. Complexation of curcumin with soy protein isolate and its implications on solubility and stability of curcumin10.1016/j.foodchem.2011.08.025 ↗
38. Interaction between silver nanoparticle and bovine hemoglobin at different temperatures10.1007/s11051-008-9538-1 ↗
39. Study of the interaction between monoammonium glycyrrhizinate and bovine serum albumin10.1016/j.jpba.2004.08.021 ↗
40. Biophysical studies on the interactions of a classic mitochondrial uncoupler with bovine serum albumin by spectroscopic, isothermal titration calorimetric and molecular modeling methods10.1007/s10895-010-0733-y ↗
41. Spectrometric study on the binding of curcumin with AOT: effect of micelle-to-vesicle transition10.1016/j.foodchem.2014.03.129 ↗
42. Fluorescence spectroscopic investigation of the interaction between chloramphenicol and lysozyme10.1016/j.ejmech.2009.04.047 ↗
43. Binding of mitomycin C to blood proteins: a spectroscopic analysis and molecular docking10.1016/j.molstruc.2009.03.016 ↗
44. Storage stability and skin permeation of vitamin C liposomes improved by pectin coating10.1016/j.colsurfb.2014.02.036 ↗
45. Preservation of catechin antioxidant properties loaded in carbohydrate nanoparticles10.1016/j.carbpol.2011.04.029 ↗
46. Singlet oxygen quenching by dietary carotenoids in a model membrane environment10.1016/S0003-9861(03)00014-6 ↗
47. The in vitro stability and in vivo pharmacokinetics of curcumin prepared as an aqueous nanoparticulate formulation10.1016/j.biomaterials.2010.04.062 ↗
48. Poly (β-cyclodextrin)/curcumin self-assembly: a novel approach to improve curcumin delivery and its therapeutic efficacy in prostate cancer cells10.1002/mabi.201000084 ↗
49. Acid pH in tumors and its potential for therapeutic exploitation
50. Interstitial pH and pO2 gradients in solid tumors in vivo: high-resolution measurements reveal a lack of correlation10.1038/nm0297-177 ↗
51. pH-sensitive nano-systems for drug delivery in cancer therapy10.1016/j.biotechadv.2013.11.009 ↗
52. Concordance of folate receptor-α expression between biopsy, primary tumor and metastasis in breast cancer and lung cancer patients10.18632/oncotarget.7856 ↗
53. A current review of folate receptor alpha as a potential tumor target in non-small-cell lung cancer
54. Overexpression of folate binding protein in ovarian cancers10.1002/(SICI)1097-0215(19970422)74:2<193::AID-IJC10>3.0.CO;2-F ↗
55. The role of folate receptor α in cancer development, progression and treatment: cause, consequence or innocent bystander?10.1002/ijc.21712 ↗