Functionalization of a chemically treated Ti6Al4V-ELI alloy with nisin for antibacterial purposes
Source abstract
Abstract
This research aims to define a protocol for nisin adsorption onto Ti6Al4V- Extra Low Interstitial content (ELI) alloy to reduce the risk of peri-implant infections. The substrate is, first, etched to get a nanotextured surface with a high density of acidic hydroxyl groups and then functionalized with the antimicrobial peptide nisin. Nisin adsorption is performed at different pH values, in the range of 5–7. The nisin release in inorganic solutions mimicking physiological or pro-inflammatory conditions is tested. The surfaces are characterized by profilometry, SEM/EDS, contact angle and surface free energy measurements, zeta potential titrations, DLS, XPS, and UV–visible spectroscopy. Effective surface adsorption was achieved and maximized at pH 6. The coated surface has high surface energy suitable for tissue integration and it releases nisin in a time longer than 1 day. As a confirmation of the antibacterial properties due to the nisin adsorption, specimens were incubated with Staphylococcus aureus, whose metabolic activity was reduced by ≈ 70% in comparison to the untreated control, and the number of viable adhered colonies was ≈ 6 times reduced. In conclusion, coupling of nisin to a chemically treated titanium surface is promising for a bioactive and antibacterial surface for tissue integration.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Dental materials and restorations · Dental Implant Techniques and Outcomes
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (69)
Show source-provided references
1. Titanium based biomaterial for bone implants: A mini review
2. Effects of pore size and porosity of surface-modified porous titanium implants on bone tissue ingrowth10.1016/S1003-6326(19)65161-7 ↗
3. HA-based coating by plasma spray techniques on titanium alloy for orthopedic applications
4. Crystal growth mechanism of calcium phosphate coatings on titanium by electrochemical deposition10.1016/j.surfcoat.2017.12.011 ↗
5. Sol gel deposited hydroxyapatite-based coating technique on porous titanium niobium for biomedical applications: A mini review
6. Antibacterial and bioactive nanostructured titanium surfaces for bone integration10.1016/j.apsusc.2014.05.056 ↗
7. Bone integration properties of antibacterial biomimetic porous titanium implants10.1016/S1003-6326(17)60225-5 ↗
8. Shaping the structure and properties of titanium and Ti6Al7Nb titanium alloy in low-temperature plasma nitriding processes10.1016/j.jallcom.2021.158896 ↗
9. Photolithographic-based stamp technique for improving the biocompatibility of antibacterial titanium implant by dynamic clearance of antibacterial agents10.1016/j.cej.2021.129756 ↗
10. Antibacterial titanium surfaces for medical implants10.1016/j.msec.2015.12.062 ↗
11. https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance - visited on 16.09.2021.
12. What’s new in mechanisms of antibiotic resistance in bacteria of clinical origin?10.1016/j.eimce.2020.02.017 ↗
13. Antibacterial metals and alloys for potential biomedical implants
14. Current Developments in Antimicrobial Surface Coatings for Biomedical Applications10.2174/0929867321666140916121355 ↗
15. Lotus-leaf-inspired hierarchical structured surface with non-fouling and mechanical bactericidal performances10.1016/j.cej.2020.125609 ↗
16. Micro-nano manufacturing of Ti6Al4V antibacterial surface10.1016/j.colsurfa.2021.126929 ↗
17. Cytocompatible and Anti-bacterial Adhesion Nanotextured Titanium Oxide Layer on Titanium Surfaces for Dental and Orthopedic Implants10.3389/fbioe.2019.00103 ↗
18. Surface structuring by Electron Beam for improved soft tissues adhesion and reduced bacterial contamination on Ti-grade 210.1016/j.jmatprotec.2018.11.026 ↗
19. Nisin—A lantibiotic with immunomodulatory properties: A review10.1016/j.peptides.2020.170479 ↗
20. Biomedical applications of nisin10.1111/jam.13033 ↗
21. Dual antibacterial mechanisms of nisin Z against Gram-positive and Gram-negative bacteria10.1016/j.ijantimicag.2005.08.010 ↗
22. The lantibiotic nisin, a special case or not?, Biochimica et Biophysica Acta (BBA) -10.1016/S0005-2736(99)00208-4 ↗
23. Effects of pH, Temperature, Storage Time, and Protective Agents on Nisin Antibacterial Stability
24. Application of oxidized metallic surfaces as a medium to store biochemical agents with antimicrobial properties10.1016/j.surfcoat.2019.05.059 ↗
25. Antifouling performance analysis of peptide-modified glass microstructural surfaces10.1016/j.apsusc.2020.148384 ↗
26. Optimized grafting of antimicrobial peptides on stainless steel surface and biofilm resistance tests10.1016/j.colsurfb.2011.01.012 ↗
27. Linker-free covalent immobilization of nisin using atmospheric pressure plasma induced grafting10.1039/C7TB00113D ↗
28. Surface Functionalization of Ti6Al4V-ELI Alloy with Antimicrobial Peptide Nisin10.3390/nano12234332 ↗
29. Evolution of plastic deformation and its effect on mechanical properties of laser additive repaired Ti64ELI titanium alloy10.1016/j.optlastec.2016.12.038 ↗
30. Multifunctional titanium surfaces for bone integration
31. Surface functionalization of Ti6Al4V with an extract of polyphenols from red grape pomace10.1016/j.matdes.2021.109776 ↗
32. Photofunctionalization of Titanium: An Alternative Explanation of Its Chemical-Physical Mechanism10.1371/journal.pone.0157481 ↗
33. Nisin infusion into surface cracks in oxide coatings to create an antibacterial metallic surface10.1016/j.msec.2019.110034 ↗
34. The corrosion resistance of pure titanium in organic acids10.1016/S0142-9612(01)00040-0 ↗
35. Influence of fluoride content and pH on the corrosion resistance of titanium and its alloys10.1016/S0142-9612(01)00328-3 ↗
36. S. Wu, Polymer interface and adhesion, 1st ed., Routledge, 1982. https://doi.org/10.1201/9780203742860.10.1201/9780203742860 ↗
37. N. Fairley, V. Fernandez, M. Richard‐Plouet, C. Guillot-Deudon, J. Walton, E. Smith, D. Flahaut, M. Greiner, M. Biesinger, S. Tougaard, D. Morgan, J. Baltrusaitis, Systematic and collaborative approach to problem solving using X-ray photoelectron spectroscopy, Applied Surface Science Advances. 5 (2021) 100112. https://doi.org/10.1016/j.apsadv.2021.100112.10.1016/j.apsadv.2021.100112 ↗
38. The effect of simulated inflammatory conditions and Fenton chemistry on the electrochemistry of CoCrMo alloy, Journal of Biomedical Materials Research Part B: Applied
39. Antibacterial, pro-angiogenic and pro-osteointegrative zein-bioactive glass/copper based coatings for implantable stainless steel aimed at bone healing
40. Competitive surface colonizaion of antibacterial and bioactive materials doped with strontium and/or silver ions10.3390/nano10010120 ↗
41. Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening10.1038/nprot.2010.71 ↗
42. The surface conditions and composition of titanium alloys in implantology: A comparative study of dental implants of different brands10.3390/ma15031018 ↗
43. R. Zahran, J.I. Rosales Leal, M.A. Rodríguez Valverde, M.A. Cabrerizo Vilchez, Effect of hydrofluoric acid etching time on titanium topography, chemistry, wettability, and cell adhesion, PLos ONE. 11 (n.d.) e0165296. https://doi.org/10.1371/journal.pone.0165296.10.1371/journal.pone.0165296 ↗
44. Enhanced antimicrobial activity of the food-protecting nisin peptide by bioconjugation with silver nanoparticles10.1007/s10311-017-0626-2 ↗
45. Preparation of extra-small nisin nanoparticles for enhanced antibacterial activity after autoclave treatment10.1016/j.foodchem.2017.11.116 ↗
46. Preparation and antimicrobial activity of pectin-chitosan embedding nisin microcapsules10.1016/j.eurpolymj.2021.110676 ↗
47. Nisin/polyanion layer-by-layer films exhibiting different mechanisms in antimicrobial efficacy10.1039/C9RA10135G ↗
48. Improvement of solubility and stability of the antimicrobial peptide nisin by protein engineering10.1128/aem.61.8.2873-2878.1995 ↗
49. Wettability studies of topologically distinct titanium surfaces10.1016/j.colsurfb.2015.03.024 ↗
50. Surface modification of titanium surfaces through a modified oxide layer and embedded silver nanoparticles: Effect of reducing/stabilizing agents on precipitation and properties of the nanoparticles10.1016/j.surfcoat.2018.03.020 ↗
51. Construction of an environmentally friendly octenylsuccinic anhydride modified pH-sensitive chitosan nanoparticle drug delivery system to alleviate inflammation and oxidative stress10.1016/j.carbpol.2020.115972 ↗
52. Molecular Dynamics Simulations of Proteins and Protein-Protein Complexes
53. Properties and Biological Significance of Natural Oxide Films on Titanium and Its Alloys
54. Surface chemistry effect of topographical modification of titanium dental implant surfaces: 1
55. Coupling of keratin with titanium: A physico-chemical characterization of functionalized or coated surfaces10.1016/j.surfcoat.2020.126057 ↗
56. High resolution X-ray photoelectron spectroscopy of l-cysteine self-assembled films10.1039/b405516k ↗
57. NIST X-ray Photoelectron Spectroscopy Database, Version 4.1 (National Institute of Standards and Technology, Gaithersburg, 2012). http://srdata.nist.gov/xps/.
58. Hollinger · 2012
59. The use of vitamin E as an anti-adhesive coating for cells and bacteria for temporary bone implants10.1016/j.surfcoat.2022.128694 ↗
60. How do wettability, zeta potential and hydroxylation degree affect the biological response of biomaterials?10.1016/j.msec.2016.12.107 ↗
61. Antimicrobial peptides10.4161/psb.6.9.16319 ↗
62. Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment10.3389/fbioe.2020.00561 ↗
63. Protease-armed bacteria in the skin, Cell and Tissue
64. Effect of sub-lethal doses of nisin on Staphylococcus aureus toxin production and biofilm formation10.1016/j.toxicon.2021.03.018 ↗
65. F.P. da Silva, K.M. Fernandes, L.L. de Freitas, R. de S. Cascardo, R.C. Bernardes, L.L. de Oliveira, G.F. Martins, M.C.D. Vanetti, Effects of sub-lethal doses of nisin on the virulence of Salmonella enterica in Galleria mellonella larvae, Res. Microbiol. 172 (2021) 103836. https://doi.org/10.1016/j.resmic.2021.103836.10.1016/j.resmic.2021.103836 ↗
66. Reduced bacterial adhesion on ceramics used for arthroplasty applications10.1016/j.jeurceramsoc.2017.10.008 ↗
67. Dual Action Antimicrobial Surfaces: Alternating Photopatterns Maintain Contact-Killing Properties with Reduced Biofilm Formation10.1002/mame.202070026 ↗
68. Polysulfone based ultrafiltration membranes with dopamine and nisin moieties showing antifouling and antimicrobial properties10.1016/j.seppur.2018.03.033 ↗
69. Nisin-based antibacterial and antiadhesive layer-by-layer coatings, Colloids and Surfaces10.1016/j.colsurfb.2021.112121 ↗