Study of poly (N-isopropyl acrylamide) electrospinning magnetic fiber as a cell attach/detach substrate

In this study, we used electrospinning technology to develop a novel composite material with temperature and magnetic responsive characteristics which physical and chemical parameters were verified. Poly(N-isopropylacrylamide), PNIPAAm polymer was chosen as the main material for the spinning process, to prepare the temperature-responsive material with a hydrophobic/hydrophilic fiber structure. The fibers prepared by the electrospinning method resulted in high pores and high volume-surface area that can serve as substrates for cell tissue engineering applications. The material was then integrated with magnetic nanoparticles which led to composite materials of magnetic temperature-responsive spinning fibers and can further be used as a substrate material in the biomedical engineering field. Field-scanning emission electron microscopy (FSEM) and transmission electron microscopy (TEM) were used to characterize the spinning fibers diameter and surface morphology. The superconducting quantum interference device (SQUID) and thermogravimetric analyzer (TGA) were used to analyze the amount of magnetic nanoparticles confined in the magnetic temperature-responsive spinning fiber. The proportion of magnetic particles in magnetic temperature-responsive spinning fiber from SQUID and TGA analysis was 3.44% and 4.07%, respectively. This study successfully established the preparation of temperature-responsive spinning fiber with extracellular matrix structure through an electrospinning technique. In the future, this composite material with the advantage of switchable hydrophilic/hydrophobic temperature-responsive and magnetic induction properties can further be used in the field of biomedical engineering applications, such as drug-carrier, drug release, skin wound healing, and magnetic high-frequency heat treatment.