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journal article·2023-07-01Open access

Spatial continuous-flow polymerase chain reaction structure controlled by single-temperature driver

Ranran Zhou, Yanguang Ding, Yu Sun, Jing Yang, Yang Yu, Jianxin Pang, Jin Chen, Fengchao Wang

AIP Advances· Volume 13· Issue 7
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Source abstract

Abstract

A spatial continuous-flow polymerase chain reaction microfluidic chip was designed with the traditional plane channel replaced by a vertical-cavity channel by a single-temperature driver. To optimize the design, the temperature distribution in the microchannel of this chip was simulated by the finite-element method with various structural parameters and fluid velocities. The results show that the fluid produces three temperature zones of 94–95, 54–56, and 71–73 °C when the chip size is 54 mm (x) × 78 mm (y) × 47 mm (z), the wall thickness is 2 mm (x) × 20 mm (y) × 2 mm (z), and the pipe diameter is 1 mm. To increase the polymerase chain reaction amplification efficiency, we considered fluid velocities in the range of 0.0005–0.002 m/s. The optimal temperature for polymerase chain reaction amplification occurs at a fluid velocity of 0.0005 m/s.
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Research context

Polymers

OpenAlex topics: Microfluidic and Capillary Electrophoresis Applications · Microfluidic and Bio-sensing Technologies · Innovative Microfluidic and Catalytic Techniques Innovation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

College of Sciences, Shanghai Institute of Technology 1 , 100 Haiquan Road, Shanghai 201418, China

Department of Thoracic Surgery, Qingpu Branch of Zhongshan Hospital, Fudan University 2 , Shanghai 201700, China

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University 3 , Suzhou, Jiangsu 215006, China

Funding

Shanghai Sailing Program, China · Research Start-up Project of Shanghai Institute of Technology · Collaborative Innovation Project of Shanghai Institute of Technology · the Science and Technology Talent Development Fund for Young and Middle-Aged Teachers of Shanghai Institute of Technology

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