Magnetic response of aggregation mixed with magnetic nanoparticles and protein for simultaneous protein detection
Source abstract
Abstract
A quick and easy method to detect the GDF-15 protein (Growth Differentiation Factor-15) has been developed, which utilize the magnetic response of magnetic nanoparticles by switching magnetic fields. The magnetic nanoparticles and GDF-15 are bound by an antigen-antibody reaction and aggregated into a spherical shape using a needle-shaped magnetic yoke. The density of GDF-15 changed as follows: 0, 1 ng/ml, 10 ng/ml, and 100 ng/ml. The increase of GDF-15 aggregated the magnetic nanoparticles and enhanced the signal-to-noise ratio. We also tried the sandwich-type bound method using a primary and secondary antibody with additional magnetic nanoparticles and obtained the enhancement of the magnetic signal in a lower concentration (under 10 ng/ml) of GDF-15. The cross-bridges between magnetic nanoparticle and the protein may strengthen the magnetic couplings of nanoparticles.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: GDF15 and Related Biomarkers · Advancements in Battery Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Graduate School of Biomedical Engineering, Tohoku University 1 , Sendai 980-8579, Japan
Department of Engineering, Tohoku University 2 , Sendai 980-8579, Japan
Funding
Japan Science and Technology Agency · Japan Agency for Medical Research and Development · Terumo Foundation for Life Sciences and Arts
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