journal article·2018-05-01Open access

Development of magnet configurations for magnetic immunostaining

Miki Kaneko, Shinichi Chikaki, Sachiko Matsuda, Akihiro Kuwahata, Masayuki Namita, Itsuro Saito, Satoshi Sakamoto, Moriaki Kusakabe, Masaki Sekino

AIP Advances· Volume 8· Issue 5

Abstract

Magnetic immunostaining using a magnet and antibody-labeled fluorescent ferrite (FF) beads is established as a rapid immunostaining. In this study, we proposed the novel configuration of magnets with the large magnetic field gradient and the strong magnetic force for magnetic immunostaining. To confirm the usefulness of the proposed magnet configuration, we performed numerical analysis of the magnetic characteristics of the proposed magnets, and the magnetic immunostaining with FF beads. It was revealed that the proposed magnets generated the strong magnetic force and promoted the immunoreaction rapidly.
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Research context

OpenAlex topics: Monoclonal and Polyclonal Antibodies Research · Glycosylation and Glycoproteins Research · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

The University of Tokyo 1 , Tokyo, Japan

Keio University 2 , Tokyo, Japan

iMed Japan Inc. 3 , Tokyo, Japan

Tokyo Institute of Technology 4 , Kanagawa, Japan

Matrix Cell Research Institute Inc. 5 , Ibaraki, Japan

Funding

Japan Agency for Medical Research and Development

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