A simple antigen-antibody reaction using ultrasmall FeCo nanoparticles
Source abstract
Abstract
In this study, we prepared ultrasmall FeCo nanoparticles (NPs) with a high magnetic moment and examined their antigen-antibody reaction for biodetection applications. The FeCo NPs were collected from the FeCo-BaF2 nanogranular film with Fe:Co:Ba:F = 14:11:21:54 at.%, by dissolving the film in water since the BaF2 matrix was deliquescent. The size of FeCo NPs was ∼5 nm and the saturation magnetization was estimated to be ∼15.30 kG (149.0 emu/g). The Candida albicans antibodies (abcam ab53891)-conjugated FeCo NPs were collected by using an ultracentrifugal separation (110 000 rpm, 90 min), they were then reacted with Candida albicans. The obtained result indicates that Candida albicans were absorbed successfully onto FeCo NPs, and the number of Candida albicans bound to FeCo NPs counted from the micrographs of the aggregates of FeCo NPs and Candida albicans increased significantly by adding sonication treatment of the film in water before binding them to the antibodies. The success of antigen-antibody reaction of ultrasmall NPs with high magnetic moment improves detection sensitivity as well as offers potential detection for smaller biomolecules.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Characterization and Applications of Magnetic Nanoparticles · Advanced biosensing and bioanalysis techniques · Nanoparticle-Based Drug Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Graduate School of Engineering, Tohoku University 1 , Miyagi 980-8579, Japan
Graduate School of Biomedical Engineering, Tohoku University 2 , Miyagi 980-8579, Japan
Research Institute for Electromagnetic Materials, Denjiken 3 , Tomiya 981-3341, Japan
Graduate School of Agricultural Science, Tohoku University 4 , Miyagi 980-8570, Japan
Funding
Ministry of Internal Affairs and Communications · High Energy Accelerator Research Organization · Adaptable and Seamless Technology Transfer Program through Target-Driven R and D · Japan Society for the Promotion of Science
References (36)
Show source-provided references
1. Magnetic iron oxide nanoparticles: Synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications10.1021/cr068445e ↗
2. Recent progress on magnetic iron oxide nanoparticles: Synthesis, surface functional strategies, and biomedical applications10.1088/1468-6996/16/2/023501 ↗
3. Biofunctional magnetic nanoparticles for protein separation and pathogen detection10.1039/b514130c ↗
4. Homogeneous biosensing based on magnetic particle labels10.3390/s16060828 ↗
5. Using magnetic field gradients to shorten the antigen-antibody reaction time for a magnetic immunoassay10.1109/tmag.2019.2894904 ↗
6. A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles10.1063/1.5130437 ↗
7. A detection and analysis of fusobacterium utilizing changes in the magnetic properties of magnetic nanoparticles-antibody–antigen aggregates10.1109/tmag.2022.3161508 ↗
8. Biosensing utilizing magnetic markers and superconducting quantum interference devices10.1088/1361-6668/aa5fce ↗
9. Giant magnetoresistance-based biosensor for detection of influenza A virus10.3389/fmicb.2016.00400 ↗
10. Quantitative determination of magnetic beads using a magnetoimpedance-based lab-on-a-chip platform10.1063/1.4882095 ↗
11. A giant magnetoresistance ring-sensor based microsystem for magnetic bead manipulation and detection10.1063/1.3536822 ↗
12. Rapid detection of Escherichia coli O157:H7 using tunneling magnetoresistance biosensor10.1063/1.4977017 ↗
13. Highly sensitive third-harmonic detection method of magnetic nanoparticles using an AC susceptibility measurement system for liquid-phase assay10.1109/tasc.2016.2581703 ↗
14. Size dependence of magnetic parameters and surface disorder in magnetite nanoparticles10.1063/1.3055272 ↗
15. Magnetic nanoparticles and magnetic particle spectroscopy-based bioassays: A 15-year recap10.1088/2399-1984/ac5cd1 ↗
16. High-moment magnetic nanoparticles10.1007/s11051-020-4758-0 ↗
17. Applications of exchange coupled bi-magnetic hard/soft and soft/hard magnetic core/shell nanoparticles10.1016/j.physrep.2014.09.007 ↗
18. Synthesis of high magnetic moment CoFe nanoparticles via interfacial diffusion in core/shell structured Co/Fe nanoparticles10.1007/s12274-009-9037-4 ↗
19. Nanomagnetics10.1088/0953-8984/15/20/202 ↗
20. Investigation of commercial iron oxide nanoparticles: Structural and magnetic property characterization10.1021/acsomega.0c05845 ↗
21. Simplified fabrication of magnetic nanoparticles with directly adsorbed antibodies for bacteria detection10.1109/tmag.2022.3168360 ↗
22. Synthesis of Fe–Co nanoparticles with high saturation magnetization by low-temperature post-annealing10.1063/1.3679027 ↗
23. Synthesis of air stable FeCo nanoparticles10.1063/1.3540387 ↗
24. Synthesis of monodisperse iron nanoparticles with a high saturation magnetization using an Fe(CO)x−oleylamine reacted precursor10.1021/jp911161g ↗
25. Structure and magnetic properties of FeCo nanoparticles encapsulated in carbon nanotubes grown by microwave plasma enhanced chemical vapor deposition10.1016/j.jmmm.2011.06.029 ↗
26. One-pot synthesis of high magnetization air-stable FeCo nanoparticles by modified polyol method10.1016/j.matlet.2012.10.019 ↗
27. Synthesis of Fe-Co based nanomagnetic materials
28. Monodisperse iron-based particles: Precipitation in liquid polyols10.1039/jm9960601047 ↗
29. Chemical synthesis of sub-micrometer-to nanometer-sized magnetic FeCo dice10.1002/adma.200601292 ↗
30. Giant Faraday rotation in metal-fluoride nanogranular films10.1038/s41598-018-23128-5 ↗
31. Giant Faraday effect of FeCo-BaF and FeCo-SiN nanogranular films10.1002/ecj.12308 ↗
32. Biomedical nanomagnetics: A spin through possibilities in imaging, diagnostics, and therapy10.1109/tmag.2010.2046907 ↗
33. Nano-particle magnetism with a dispersion of particle sizes10.1063/1.4766817 ↗
34. Influence of spark plasma sintering parameters on magnetic properties of FeCo alloy10.1063/1.4993529 ↗
35. Size-dependent magnetic and inductive heating properties of Fe3O4 nanoparticles: Scaling laws across the superparamagnetic size10.1039/c7cp08631h ↗
36. Static and dynamic magnetic properties of spherical magnetite nanoparticles10.1063/1.1599959 ↗