Spectral behavior of second harmonic signals from organic and non-organic materials in multiphoton microscopy
Source abstract
Abstract
Multimodal nonlinear microscopy allows imaging of highly ordered biological tissue due to spectral separation of nonlinear signals. This requires certain knowledge about the spectral distribution of the different nonlinear signals. In contrast to several publications we demonstrate a factor of 122 relating the full width at half maximum of a gaussian laser pulse spectrum to the corresponding second harmonic pulse spectrum in the spatial domain by using a simple theoretical model. Experiments on monopotassium phosphate crystals (KDP-crystals) and on porcine corneal tissue support our theoretical predictions. Furthermore, no differences in spectral width were found for epi- and trans-detection of the second harmonic signal. Overall, these results may help to build an optimized multiphoton setup for spectral separation of nonlinear signals.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Advanced Fluorescence Microscopy Techniques · Spectroscopy Techniques in Biomedical and Chemical Research · Optical Coherence Tomography Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Friedrich-Schiller-University Jena 1 Institute of Applied Optics, , Froebelstieg 1, 07743 Jena, Germany
University of Rostock 2 Institute for Physics, Semiconductor Optics Group, , 18055 Rostock, Germany
University of Rostock 3 Department of Ophthalmology, , 18057 Rostock, Germany
Institut fuer Refraktive und Ophthalmo-Chirurgie (IROC) 4 , Stockerstrasse 37, 8002 Zuerich, Switzerland
University of Hanover 5 Institute of Quantum Optics, , 30167 Hanover, Germany
References (22)
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