3D engineered neural co-culture model and neurovascular effects of marine fungi-derived citreohybridonol
Source abstract
Abstract
Marine-based biomolecules are emerging metabolites that have gained attention for developing novel biomaterials, drugs, and pharmaceutical in vitro platforms. Here, we developed a 3D engineered neural co-culture model via a 3D prototyped sliding frame-platform for multi-step UV lithography and investigated the neurovascular potential of citreohybridonol in neuroblastoma treatment. Citreohybridonol was isolated from a sponge-derived fungus Penicillium atrovenetum. The model was characterized by Fourier-transform infrared spectroscopy and scanning electron microscopy analysis. Human umbilical cord vein endothelial cells (HUVECs) and neuroblastoma (SH-SY5Y) cell lines were encapsulated in gelatin methacrylate (GelMA) with and without citreohybridonol. The effect of citreohybridonol on the proliferation capacity of cells was assessed via cell viability and immunostaining assays. GelMA and 3D culture characterization indicated that the cells were successfully encapsulated as axenic and mixed with/without citreohybridonol. The cytotoxic test confirmed that the 3D microenvironment was non-toxic for cultural experiments, and it showed the inhibitory effects of citreohybridonol on SH-SY5Y cells and induced the proliferation of HUVECs. Finally, immunohistochemical staining demonstrated that citreohybridonol suppressed SH-SY5Y cells and induced vascularization of HUVECs in mixed 3D cell culture.
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Research context
OpenAlex topics: 3D Printing in Biomedical Research · Cellular Mechanics and Interactions · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Medicine, Department of Biophysics, KUTTAM, Koc University 1 , 34450 Istanbul, Turkey
Graduate School of Nanoscience and Nanotechnology, Department of Natural and Applied Sciences, Izmir Katip Celebi University 2 , 35620 Izmir, Turkey
EBILTEM, Ege University Science and Technology Centre 3 , 35100 İzmir, Turkey
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University 4 , 35516 Mansoura, Egypt
Department of Mechanical Engineering 5 , Koc University, 34450 Istanbul, Turkey
Anatomy Department, School of Medicine, Ege University 6 , 35040 Izmir, Turkey
Koc University Arcelik Research Center for Creative Industries (KUAR) 7 , Koc University, 34450 Istanbul, Turkey
Koc University Is Bank Artificial Intelligence Lab (KUIS AI Lab) 8 , Koc University, 34450 Istanbul, Turkey
Bogazici Institute of Biomedical Engineering, Bogazici University 9 , 34684 Istanbul, Turkey
Koc University Translational Medicine Research Center (KUTTAM), Koc University 10 , 34450 Istanbul, Turkey
Funding
Alexander von Humboldt Research Fellowship for Experienced Researchers · Tubitak 2232 International Fellowship for Outstanding Researchers Award · Marie Sklodowska-Curie Individual Fellowship · Royal Academy Newton-Katip Celebi Transforming Systems Through Partnership Award · Science Academy's Young Scientist Awards Program · Outstanding Young Scientists Awards · Bilim Kahramanlari Dernegi The Young Scientist Award
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