journal article·2018-05-01Open access

PIEZO channel protein naturally expressed in human breast cancer cell MDA-MB-231 as probed by atomic force microscopy

Yuanqi Weng, Fei Yan, Runkang Chen, Ming Qian, Yun Ou, Shuhong Xie, Hairong Zheng, Jiangyu Li

AIP Advances· Volume 8· Issue 5

Abstract

Mechanical stimuli drives many physiological processes through mechanically activated channels, and the recent discovery of PIEZO channel has generated great interests in its mechanotransduction. Many previous researches investigated PIEZO proteins by transcribing them in cells that originally have no response to mechanical stimulation, or by forming PIEZO-combined complexes in vitro, and few studied PIEZO protein’s natural characteristics in cells. In this study we show that MDA-MB-231, a malignant cell in human breast cancer cell line, expresses the mechanosensitive behavior of PIEZO in nature without extra treatment, and we report its characteristics in response to localized mechanical stimulation under an atomic force microscope, wherein a correlation between the force magnitude applied and the channel opening probability is observed. The results on PIEZO of MDA-MB-231 can help establish a basis of preventing and controlling of human breast cancer cell via mechanical forces.
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Research context

OpenAlex topics: Erythrocyte Function and Pathophysiology · Blood properties and coagulation · Ion channel regulation and function

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory of Low Dimensional Materials and Application Technology of Ministry of Education, School of Materials Science and Engineering, Xiangtan University 1 , Xiangtan 411105, Hunan, China

Shenzhen Key Laboratory of Nanobiomechanics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences 2 , Shenzhen 518055, Guangdong, China

Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences 3 , Shenzhen 518055, Guangdong, China

Hunan Provincial Key Laboratory of Health Maintenance for Mechanical Equipment, Hunan University of Science and Technology 4 , Xiangtan 411201, Hunan, China

Department of Mechanical Engineering, University of Washington 5 , WA 98195, Seattle, USA

Funding

National Key Research and Development Program of China · the Leading Talents Program of Guangdong Province · National Natural Science Foundation of China

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