Determination of electric field at and near the focus of a cylindrical lens for applications in fluorescence microscopy
Source abstract
Abstract
We present an explicit computable integral solution of the electric field generated at the focal region of a cylindrical lens. This representation is based on vectorial diffraction theory and further enables the computation of the system point spread function of a cylindrical lens. It is assumed that there is no back-scattering and the contribution from the evanescent field is negligible. Stationary phase approximation along with the Fresnel transmission coefficients are employed for evaluating the polarization dependent electric field components. Studies were carried out to determine the polarization effects and to calculate the system resolution. The effect of s −, p − and randomly polarized light is studied on the fixed sample (electric dipole is fixed in space). Proposed approach allows better understanding of electric field effects at the focus of a cylindrical aplanatic system. This opens up future developments in the field of fluorescence microscopy and optical imaging.
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Research context
OpenAlex topics: Near-Field Optical Microscopy · Advanced Fluorescence Microscopy Techniques · Digital Holography and Microscopy
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Indian Institute of Science 1 Department of Instrumentation and Applied Physics, , Bangalore 560012, India
Indian Institute of Science 2 Applied Photonics Initiative, , Bangalore 560012, India
References (38)
Show source-provided references
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