Wide field-of-view lensless fluorescence imaging device with hybrid bandpass emission filter
Source abstract
Abstract
We demonstrate a highly sensitive lensless fluorescence imaging device with a wide field-of-view by using a hybrid bandpass filter composed of interference filters, an absorption filter, and a fiber optic plate. The hybrid filter shows high excitation light rejection characteristics even in a lensless setup. In this study, we fabricated a hybrid bandpass filter and improved fluorescence observation performance for a target with auto-fluorescence. The filter was combined with a large image sensor with an imaging area of 67 mm2. As a demonstration, a brain slice from a green fluorescent protein transgenic mouse was observed and fluorescent cell bodies were detected with the lensless imaging device.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Advanced Fluorescence Microscopy Techniques · Optical Coherence Tomography Applications · Photoacoustic and Ultrasonic Imaging
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology 8916-5 Takayama , Ikoma, Nara 630-192, Japan
Funding
Support Center for Advanced Telecommunications Technology Research Foundation · Japan Society for the Promotion of Science · Core Research for Evolutional Science and Technology · Murata Science Foundation
References (35)
Show source-provided references
1. Miniaturized lensless imaging systems for cell and microorganism visualization in point-of-care testing10.1002/biot.201000427 ↗
2. Imaging without lenses: Achievements and remaining challenges of wide-field on-chip microscopy10.1038/nmeth.2114 ↗
3. Lensless imaging and sensing10.1146/annurev-bioeng-092515-010849 ↗
4. The epetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM)10.1073/pnas.1110681108 ↗
5. Wide field-of-view on-chip talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator10.1021/ac303356v ↗
6. A CMOS/thin-film fluorescence contact imaging microsystem for DNA analysis10.1109/tcsi.2010.2043990 ↗
7. 4.2 a fully integrated CMOS fluorescence biochip for multiplex polymerase chain-reaction (PCR) processes
8. Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope10.1039/c1lc20654k ↗
9. Wide and scalable field-of-view talbot-grid-based fluorescence microscopy10.1364/ol.37.005018 ↗
10. Low light CMOS contact imager with an integrated poly-acrylic emission filter for fluorescence detection10.3390/s100505014 ↗
11. A PVAc-based benzophenone-8 filter as an alternative to commercially available dichroic filters for monitoring calcium activity in live neurons via Fura-2 am10.1109/jphot.2012.2200668 ↗
12. Lensless miniature portable fluorometer for measurement of chlorophyll and CDOM in water using fluorescence contact imaging10.1109/jphot.2014.2326665 ↗
13. Optofluidic ultrahigh-throughput detection of fluorescent drops10.1039/c4lc01465k ↗
14. Micro-light-pipe array with an excitation attenuation filter for lensless digital enzyme-linked immunosorbent assay10.7567/jjap.55.03df03 ↗
15. On-chip cell analysis platform: Implementation of contact fluorescence microscopy in microfluidic chips10.1063/1.4986872 ↗
16. Highly sensitive lens-free fluorescence imaging device enabled by a complementary combination of interference and absorption filters10.1364/boe.9.004329 ↗
17. Lensless wide-field fluorescent imaging on a chip using compressive decoding of sparse objects10.1364/oe.18.010510 ↗
18. Lensfree fluorescent on-chip imaging of transgenic caenorhabditis elegans over an ultra-wide field-of-view10.1371/journal.pone.0015955 ↗
19. Wide-field lensless fluorescent microscopy using a tapered fiber-optic faceplate on a chip10.1039/c0an00926a ↗
20. Spectral demultiplexing in holographic and fluorescent on-chip microscopy10.1038/srep03760 ↗
21. Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations10.1038/nbt.1641 ↗
22. Large-scale femtoliter droplet array for digital counting of single biomolecules10.1039/c2lc40632b ↗
23. A CMOS image sensor with stacked photodiodes for lensless observation system of digital enzyme-linked immunosorbent assay10.7567/jjap.53.04el02 ↗
24. Compact lensless fluorescence counting system for single molecular assay10.1109/tbcas.2018.2820151 ↗
25. Complementary metal–oxide–semiconductor image sensor with microchamber array for fluorescent bead counting10.1143/jjap.51.02bl01 ↗
26. Wide field-of-view talbot grid-based microscopy for multicolor fluorescence imaging10.1364/oe.21.014555 ↗
27. Single-frame 3D fluorescence microscopy with ultraminiature lensless flatscope10.1126/sciadv.1701548 ↗
28. Dual-mode lensless imaging device for digital enzyme linked immunosorbent assay
29. Implantable CMOS biomedical devices10.3390/s91109073 ↗
30. Intravital fluorescence imaging of mouse brain using implantable semiconductor devices and epi-illumination of biological tissue10.1364/boe.6.001553 ↗
31. Implantable micro-optical semiconductor devices for optical theranostics in deep tissue10.7567/apex.9.047001 ↗
32. Implantable imaging device for brain functional imaging system using flavoprotein fluorescence10.7567/jjap.55.03df02 ↗
33. Implantable microimaging device for observing brain activities of rodents10.1109/jproc.2016.2585585 ↗
34. Miniaturized integration of a fluorescence microscope10.1038/nmeth.1694 ↗
35. A wireless fiber photometry system based on a high-precision CMOS biosensor with embedded continuous-time ΣΔ modulation10.1109/tbcas.2018.2817200 ↗