CCT2 Promotes Prostate Cancer Progression Through EIF3F‐Dependent Stabilization of FASN

ABSTRACT Prostate cancer (PCa) is increasingly recognized to be driven by dysregulated lipid metabolism. Although fatty acid synthase (FASN) is highly expressed in PCa, the mechanisms governing FASN protein stability and its functional integration into oncogenic lipid metabolism remain poorly defined. In this study, we identified chaperonin‐containing TCP1 subunit 2 (CCT2) as a key oncogenic regulator that promotes lipid synthesis and enhances malignant phenotypes both in vitro and in vivo. Mechanistically, CCT2 transcription is upregulated by the transcription factor Forkhead Box A1 (FOXA1); the CCT2 protein interacts with eukaryotic translation initiation factor 3 subunit F (EIF3F) and FASN to facilitate the assembly of a CCT2/EIF3F/FASN ternary complex. This complex enhances the EIF3F‐mediated deubiquitination of FASN, increasing FASN stability and lipid synthesis, and accelerating tumor progression. Either orlistat‐mediated FASN inhibition or Y043‐8015‐induced disruption of the CCT2‐EIF3F interaction effectively suppressed CCT2‐driven tumor progression in vivo. Importantly, combined treatment produced synergistic antitumor effects, significantly reducing tumor growth and metastatic burden across multiple in vivo models, including isograft and patient‐derived xenograft models. This study reveals that CCT2 promotes lipid metabolic reprogramming and tumor progression in prostate cancer by cooperating with EIF3F to stabilize FASN, highlighting the CCT2‐EIF3F‐FASN axis as a potential target for metabolic intervention.