Resistant Peanut Genotype Reprograms Rhizosphere Metabolism to Enhance Bacterial Wilt Suppression

ABSTRACT Bacterial wilt caused by Ralstonia solanacearum compromises the yield and quality of peanut ( Arachis hypogaea L.). While rhizosphere microbiome‐assisted defense is known, how resistant plant genotypes orchestrate this process remains unclear. Here, we integrate multi‐omics analyses of resistant and susceptible peanut genotypes to uncover a genotype‐specific defense mechanism. The resistant genotype selectively recruits beneficial bacteria (e.g., Kosakonia and Frankia ), which coincides with activated salicylic acid (SA)‐dependent systemic acquired resistance (SAR). Crucially, we identify keystone rhizosphere metabolites (including betaine, arginine, and SA) that are positively correlated with both beneficial microbiome assembly and SAR gene expression, establishing a self‐reinforcing defense loop. Leveraging these insights, we develop a prebiotic formulation that enhances beneficial microbial recruitment and stimulates SAR. Field trials demonstrate that the prebiotics reduce bacterial wilt incidence from 84.2% to 5.0% and increase yield by 12.9%–20.3%. Collectively, our study reveals a synergistic microbiome‐immune co‐regulation mechanism in peanut and delivers a translatable solution for sustainable disease management.