Ultrasound Modulation of Visual Circuits in Mice Independent of Auditory Confound
Source abstract
Abstract
ABSTRACT Despite its growing potential as a noninvasive neuromodulation technology, the fundamental biophysical mechanism of focused ultrasound neuromodulation (FUN) remains elusive. Progress has been hampered by a persistent auditory confound, making it difficult to isolate the direct mechanical effects on neural circuits. To overcome this barrier, we employed two‐photon calcium imaging (2PCI) in the primary visual cortex (V1) of deafened mice. Here, this study identifies a sparse (<1.5%) and spatially distributed population of neurons that is robustly and directly activated by ultrasound in a pressure‐dependent manner. Crucially, this sparse, direct activation is sufficient to modulate the broader visual circuit, altering the response dynamics of neighboring neurons to visual stimuli. By unequivocally decoupling direct neuromodulation from auditory artifacts, the findings identify a key cellular substrate for FUN and establish a robust framework for future studies. This work paves the way for targeted investigations into the precise biophysical mechanisms governing the interaction between ultrasound and neurons.
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Research context
OpenAlex topics: Ultrasound and Hyperthermia Applications · Ultrasound and Cavitation Phenomena · Photoacoustic and Ultrasonic Imaging
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Guangdong Institute of Intelligence Science and Technology Zhuhai Guangdong P. R. China
Faculty of Health Sciences/Centre For Cognitive and Brain Sciences University of Macau Macao SAR P. R. China
Department of Biomedical Engineering The Hong Kong Polytechnic University Hung Hom Hong Kong SAR P. R. China
Department of Neurosurgery Huashan Hospital Shanghai Medical College National Center for Neurological Disorders Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration Shanghai Clinical Medical Center of Neurosurgery Fudan University, Neurosurgical Institute of Fudan University Department of Neurosurgery & Neurocritical Care Huashan Hospital Shanghai Medical College Fudan University Shanghai P. R. China
Funding
National Natural Science Foundation of China · Natural Science Foundation of Shanghai Municipality · Hong Kong Polytechnic University
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