DataVXAdvanced SciencePaper
journal article·2026-03Open access

Dynamic Decoration of DNA Scaffolds for High‐Resolution Cancer Cell Subtyping

Xiaolin Hu, Jie Xie, Xinlin Guo, Liangting Wang, Zhengheng Yu, Xiaopei Qiu, Heng Li, Kang Wang, Xiaoxing Wang, Mingxuan Song, Junsong Guo, Wei Gu, Sergio Bernardini, Chaoyong Yang, Hong Zhang, Yang Luo

Advanced Science· Volume 13· Issue 17
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Source abstract

Abstract

ABSTRACT Precise imaging of cancer cells serves as the foundation for subtype analysis, significantly advancing the development of precision medicine. The unavoidable cellular internalization of fluorescent labels constrains the resolution and timeliness, presenting a significant obstacle. This study introduces a dynamic decorating strategy of DNA scaffolds that enables the execution of four distinct Boolean logic operations through self‐assembly and self‐disassembly. By integrating the in‐built molecular circuit, the proposed assay achieved signal‐amplified detection of low‐abundance nucleic acid inputs. In the application of cell imaging, inputs were labeled with aptamers to operate membrane‐confined self‐assembly or self‐disassembly of DNA scaffolds on the cell surface, enabling simultaneous identification of distinct subtypes of cancer cells with high fidelity. The intrinsic durability of DNA scaffolds successfully prevented cellular internalization for up to 300 min, boosting the long‐duration imaging. Moreover, the assay was capable of profiling a broad spectrum of cancer cell abundances from as low as 0.1% to 10% in clinical blood samples, consistently achieving recognition efficiency exceeding 60%. These findings underscore the transformational potential of DNA scaffold‐based imaging tools in biological research and precision medicine.
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Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · DNA and Biological Computing

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University Chongqing P. R. China

College of Bioengineering Key Laboratory For Biorheological Science and Technology of Ministry of Education Chongqing University Chongqing P. R. China

Institute of Pathology and Southwest Cancer Center Southwest Hospital Third Military Medical University (Army Medical University) and Key Laboratory of Tumor Immunopathology Ministry of Education of China Chongqing P. R. China

College of Life Science and Laboratory Medicine Kunming Medical University Kunming Yunnan P. R. China

Department of Experimental Medicine University of Rome Tor Vergata Rome Italy

The State Key Laboratory of Physical Chemistry of Solid Surfaces MOE Key Laboratory of Spectrochemical Analysis & Instrumentation Key Laboratory for Chemical Biology of Fujian Province Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen P. R. China

Funding

National Major Science and Technology Projects of China · National Natural Science Foundation of China · Natural Science Foundation of Chongqing Municipality · Chongqing Municipal Science and Technology Bureau · Major Science and Technology Projects in Yunnan Province

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