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journal article·2026-01Open access

Subventricular Zone‐on‐a‐Chip: A Model to Study Neurogenesis Disruption in Neonatal Intraventricular Hemorrhage

Laura Nicoleti Zamproni, Begüm Gökçe, Magnus Gram, Coco Holliday, Aylin Sendemir, Marimélia Aparecida Porcionatto, Anna Herland

Advanced Science· Volume 13· Issue 3
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Abstract

Abstract Intraventricular hemorrhage (IVH) in preterm infants disrupts neurogenesis in the subventricular zone (SVZ), a key neurogenic niche, yet no effective treatments exist. This work develops a human SVZ‐on‐a‐chip model to investigate the inflammatory response in IVH and its impact on neurogenesis. Using this platform, this work examines the effects of red blood cell lysate (RBCL) and hemorrhagic cerebrospinal fluid (CSF) from preterm infants with IVH on SVZ cells. Transcriptomic analysis reveal activation of inflammatory pathways in fetal astrocytes and brain microvascular endothelial cells exposed to hemoglobin isoforms. Notably, interleukin‐1B (IL1B) is upregulated following RBCL and hemorrhagic CSF exposure. To probe its role, this work applies an IL1 receptor antagonist, which demonstrate that IL1B has a partially protective influence on neurogenesis. These findings highlight the SVZ‐on‐a‐chip as a powerful tool for studying IVH pathology and emphasize the role of inflammation in regulating neurogenesis. IL1B emerges as a potential therapeutic target, offering new avenues for intervention. This study advances the understanding of IVH and lays the groundwork for developing strategies to protect the developing brain.
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Research context

OpenAlex topics: Neonatal and fetal brain pathology · Fetal and Pediatric Neurological Disorders · Cerebrospinal fluid and hydrocephalus

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biochemistry Escola Paulista de Medicina Universidade Federal de São Paulo São Paulo 04044‐020 Brazil

AIMES – Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology Stockholm 17164 Sweden

Department of Bioengineering Graduate School of Natural and Applied Sciences Ege University Izmir 35040

Pediatrics Department of Clinical Sciences Lund Lund University Lund 22184 Sweden

Department of Neonatology Skåne University Hospital Lund 22184 Sweden

Biofilms – Research Center for Biointerfaces Department of Biomedical Science Faculty of Health and Society Malmö University Malmö 20506 Sweden

Department of Immunology Harvard Medical School Boston MA 02115 USA

Department of Bioengineering Faculty of Engineering Ege University Izmir 35040 Türkiye

Department of Biomedical Technologies Graduate School of Natural and Applied Sciences Ege University Izmir 35040 Türkiye

Division of Nanobiotechnology SciLifeLab, Department of Protein Science KTH Royal Institute of Technology Solna 17165 Sweden

Department of Neuroscience Karolinska Institutet Solna 17164 Stockholm Sweden

Funding

Crafoordska Stiftelsen

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