An Efficient CO 2 ‐Upcycling Platform Based on Engineered Halomonas TD with Enhanced Acetate‐Utilizing Capacity via Adaptive Laboratory Evolution
Source abstract
Abstract
Abstract Biohybrid conversion of carbon dioxide (CO 2 ) into value‐added bioproducts via engineered microbes using CO 2 ‐derived electrolytes (CDE) addresses global CO 2 emissions, but most recombinants have poor saline CDE tolerance and low carbon conversion rate (CCR). Herein, Halomonas TD (salt‐resistant) was adaptively evolved into TD80, which efficiently uses acetate; its aceE gene mutation (encoding pyruvate dehydrogenase) drives acetate utilization. Subsequently, different biosynthesis pathways in TD80 enabled high yields of poly‐3‐hydroxybutyrate (PHB), poly‐3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate (P34HB), 3‐hydroxybutyrate (3HB), violacein, ectoine, 1,3‐diaminopropane (1,3‐DAP) and superoxide dismutase (SOD), respectively. Moreover, 26.0 g L −1 ectoine and 29.6 g L −1 PHB can be achieved by recombinant TD80 strains during fed‐batch studies. Finally, a non‐canonical pathway was designed to recycle the excess malonyl‐CoA into PHB. The resultant PHB content in fed‐batch study was increased from 60 wt% to 80 wt%. Moreover, co‐producing ectoine and PHB could further boost the CCR of CDE‐to‐product up to 53.7 mol%, which exemplified promising potential for biohybrid CO 2 upcycling involved in carbon capture and utilization system. Furthermore, TD80 was engineered to grow on formate only aiming to achieve the full use of CDE. The establishment of technology and economy assessment (TEA) confirmed the Halomonas ‐based platform’s efficiency and economic viability for carbon footprint reduction.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Microbial Metabolic Engineering and Bioproduction · CO2 Reduction Techniques and Catalysts · Microbial Fuel Cells and Bioremediation
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Biology and Biological Engineering South China University of Technology Guangzhou 510006 China
Food and Drug Department Liaoning Agricultural Vocational and Technical College Yingkou 115009 China
Department of General Surgery (Colorectal Surgery) The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou 510655 China
Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou 510655 China
Biomedical Innovation Center The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou 510655 China
School of Science China University of Geosciences Beijing 100083 China
CAS Key Laboratory of Quantitative Engineering Biology Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advanced Technology Chinese Academy of Science Shenzhen 518055 China
Guangzhou Medpha Biotechnology Co., Ltd. Guangzhou 510006 China
Guangdong Hefeng Biotechnology Co., Ltd. Zhanjiang 524300 China
China Petroleum Engineering and Construction Corporation Beijing 100120 China
Powered Carbon Biotechnology Co., Ltd Shenzhen 518055 China
Funding
National Natural Science Foundation of China · Fundamental Research Funds for the Central Universities · Shenzhen Science and Technology Innovation Program · Guangdong Provincial Department of Science and Technology
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