Material‐to‐Application Integration: Rapid Fabrication of Field‐Deployable Hydrogel‐SiO ₂ DNA Separator for Low‐Resource Point‐of‐Care Diagnostics

Abstract Current nucleic acid (NA) diagnostics are hindered in resource‐limited settings by equipment needs and high costs. S(PAA‐SiO ₂ ) is developed, a hydrogel‐SiO ₂ composite deoxyribonucleic acid (DNA) separator addressing these challenges through rapid (<10 min), simple, and skill‐free preparation, ultralow cost ($0.028 per unit), and equipment‐minimized mass production capacity (96 units per batch). Further systematic investigation of SiO ₂ particle surface modification revealed critical enhancements in DNA adsorption properties. With maintaining structural thermal stability, SiO ₂ integration significantly improved the surface roughness, specific surface area, and hydrophobicity, leading to hydrophobic and salt bridge effect‐enhanced DNA adsorption. Especially, SiO ₂ modification with mixed particle size formed a hierarchical microstructure to promote turbulence and interface interaction. Device S(PAA‐SiO ₂ ‐Mix) achieved a higher DNA extraction yield than the commercial kits. It possesses potential value for Polymerase Chain Reaction (PCR) diagnosis of bacterial, viral, parasitic, and fungal pathogens. By orchestrating three technological progresses ‐ rapid S(PAA‐SiO ₂ ‐Mix) fabrication, high‐throughput DNA extraction, and visual loop‐mediated isothermal amplification (LAMP) ‐ a field‐deployable diagnostic workflow is established for Vibrio parahaemolyticus (v. parahaemolyticus, Vpa) . This platform achieves equipment‐minimized point‐of‐care detection in <40 min with a sensitivity of 10 CFU mL ⁻¹ . Its simplicity, speed, and accuracy offer a transformative solution for resource‐limited diagnostics. This work advances separation interface design and presents a novel vision for integrated, equipment‐free molecular detection systems.