Outermost Cationic Surface Charge of Layer‐by‐Layer Films Prevents Endothelial Cells Migration for Cell Compartmentalization in Three‐Dimensional Tissues (Adv. Sci. 19/2025)

Cationic Nanofilm Facilitates Vascular Tissue Engineering Replicating complex, well-organized tissues in vitro still remains challenging. In article number 2417538, Michiya Matsusaki and co-workers report a multilayered nanofilm with a cationic outermost surface that mimics the basement membrane's barrier function, effectively inhibiting endothelial cell migration and sprouting. A stable, well-integrated 3D patterned vascular microchannel was successfully constructed with the support of shape-adaptable nanofilms, providing a promising platform for pharmacokinetic studies.