Mapping Nanoscale‐To‐Single‐Cell Phosphoproteomic Landscape by Chip‐DIA (Adv. Sci. 1/2025)

Single-Cell Phosphoproteomic Analysis In article 2402421, Hsiung-Lin Tu, Yu-Ju Chen, and co-workers present a novel phosphoproteomic chip combined with mass spectrometry to map the phosphoproteomic landscape at single-cell resolution. This cover artwork highlights the chip, symbolized as a ‘vessel’ that navigates through single-cell capture, lysis, protein digestion, and phospho-enrichment, revealing the first phosphoprotein network that drives cellular signaling within a single cell.