Development and Application of Reversible and Irreversible Covalent Probes for Human and Mouse Cathepsin‐K Activity Detection, Revealing Nuclear Activity (Adv. Sci. 38/2024)

Activity-Based Probes The cover highlights a novel chemical probe that binds a protease, cathepsin K (K), a key player in bone resorption. The probe detects active K in the cell by producing a fluorescent signal. In article number 2401518, Galia Blum and co-workers describe the development and applications of the probe, also revealing cathepsin K's activity in the nucleoli of multi-nucleated osteoclast cells.