Photoactivatable Nanobody Conjugate Dimerizer Temporally Resolves Tiam1‐Rac1 Signaling Axis (Adv. Sci. 11/2024)

Photochemical Biology Chengjian Zhou, Huiping He, and Xi Chen have developed nanobody-based photoinducible chemical dimerizer called PANCID, which combines the advantages of nanobody and photoactivatable chemically induced dimerization (pCID). Through the use of PANCID, the authors conducted an investigation on the dynamics of the Tiam1-Rac1 signaling cascade. The findings shown in article number 2307549 reveal that Rac1 and Tiam1 demonstrate distinct behaviors, functioning as time-resolved “molecular oscillators” when activated either slowly or rapidly. Additionally, the authors uncover a condition in which Tiam1 triggers cell apoptosis.