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journal article·2020-02Open access

High‐Throughput Imaging: Fast, 3D Isotropic Imaging of Whole Mouse Brain Using Multiangle‐Resolved Subvoxel SPIM (Adv. Sci. 3/2020)

Jun Nie, Sa Liu, Tingting Yu, Yusha Li, Junyu Ping, Peng Wan, Fang Zhao, Yujie Huang, Wei Mei, Shaoqun Zeng, Dan Zhu, Peng Fei

Advanced Science· Volume 7· Issue 3
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Source abstract

Abstract

In article number 1901891, Dan Zhu, Peng Fei, and co-workers develop an imaging technique, multiangle-resolved subvoxel selective plane illumination microscopy (termed Mars-SPIM), that can readily obtain the 3D digital atlas of a cleared whole mouse brain with an isotropic single-cell resolution, in a short acquisition time of several minutes. It provides an efficient way to implement system-level cellular analysis, such as the mapping of different neuron populations and tracing of long-distance neural projections over the entire brain.
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Research context

OpenAlex topics: Advanced Fluorescence Microscopy Techniques

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Affiliations

School of Optical and Electronic Information‐Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 China

Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 China

MoE Key Laboratory for Biomedical Photonics Huazhong University of Science and Technology Wuhan 430074 China

Department of Anesthesiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

References

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