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journal article·2022-11Open access

Superanionic Solvent‐Free Liquid Enzymes Exhibit Enhanced Structures and Activities

Ye Zhou, Jannik Nedergaard Pedersen, Jacob Nedergaard Pedersen, Nykola C. Jones, Søren Vrønning Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Renjun Gao, Zheng Guo

Advanced Science· Volume 9· Issue 32
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Source abstract

Abstract

Abstract The surface of a carboxylate‐enriched octuple mutant of Bacillus subtilis lipase A (8M) is chemically anionized to produce core (8M)‐shell (cationic polymer surfactants) bionanoconjugates in protein liquid form, which are termed anion‐type biofluids. The resultant lipase biofluids exhibit a 2.5‐fold increase in hydrolytic activity when compared with analogous lipase biofluids based on anionic polymer surfactants. In addition, the applicability of the anion‐type biofluid using Myoglobin (Mb) that is well studied in anion‐type solvent‐free liquid proteins is evaluated. Although anionization resulted in the complete unfolding of Mb, the active α‐helix level is partially recovered in the anion‐type biofluids, and the effect is accentuated in the cation‐type Mb biofluids. These highly active anion‐type solvent‐free liquid enzymes exhibit increased thermal stability and provide a new direction in solvent‐free liquid protein research.
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Research context

Polymers

OpenAlex topics: Enzyme Catalysis and Immobilization · Analytical Chemistry and Chromatography · Surfactants and Colloidal Systems

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory for Molecular Enzymology and Engineering The Ministry of Education School of Life Sciences Jilin University No. 2699, Qianjin Street Changchun 130012 P. R. China

Department of Biological and Chemical Engineering Aarhus University Gustav Wieds Vej 10 Aarhus 8000 Denmark

Department of Chemistry and Interdisciplinary Nanoscience Center (iNANO) Aarhus University Gustav Wieds Vej 14 Aarhus 8000 Denmark

ISA Department of Physics and Astronomy Aarhus University Ny Munkegade 120 Aarhus 8000 Denmark

Department of Biomedicine Aarhus University Wilhelm Meyers Allé 4 Aarhus 8000 Denmark

School of Cellular and Molecular Medicine University of Bristol BS8 1TS Bristol UK

Funding

Novo Nordisk Fonden

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