journal article·2025-04Open access

Structural Basis and Mechanism of a Unique Haemophore in the Haem‐Iron Acquisition by Riemerella anatipestifer

Mengying Wang, Dandan Zhang, Xiu Tian, Jiangyang Tong, Yizhou Yao, Mingshu Wang, Dekang Zhu, Renyong Jia, Shun Chen, Xinxin Zhao, Shaqiu Zhang, Juan Huang, Xumin Ou, Bin Tian, Di Sun, Yu He, Zhen Wu, Songying Ouyang, Mafeng Liu, Anchun Cheng

Advanced Science· Volume 12· Issue 13

Abstract

Abstract Several bacterial pathogens employ haemophores to scavenge haem from host haemoprotein to obtain an iron source. However, no homologues of well‐characterized haemophores are found in Riemerella anatipestifer, a bacterium belonging to the order Flavobacteriales that encodes haem uptake systems. Herein, a unique haemophore RhuH is characterized in this bacterium. R. anatipestifer used RhuH to grow when duck hemoglobin serves as the sole iron resource. RhuH is secreted as a component of outer membrane vesicles. Recombinant RhuH exhibited a high binding affinity for haem (Kd of 3.44 × 10−11 m) and can extract haem from duck hemoglobin. X‐ray crystallography elucidated the 3D structure of RhuH at 2.85 Å resolution, showing a dimeric conformation with each monomer exhibiting a unique structure. Structure modeling of RhuH‐haem, coupled with mutagenesis, haemin utilization, and binding affinity assays, show that haem is captured in the β‐barrel–like region, displaying the classic iron coordination. The RhuH homologues are predominantly distributed in Weeksellaceae and Flavobacteriaceae. Finally, the homologues of RhuH in Riemerella columbina, Flavobacterium columnare, and Flavobacterium soli are used as a proof of concept, demonstrating that these homologues exhibit conserved structures and functions.
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Research context

OpenAlex topics: Hemoglobin structure and function · Porphyrin Metabolism and Disorders · Bacterial Genetics and Biotechnology

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Engineering Research Center of Southwest Animal Disease Prevention and Control Technology Ministry of Education of the People's Republic of China Chengdu 611130 China

Key Laboratory of Animal Disease and Human Health of Sichuan Province Chengdu 611130 China

International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province Chengdu 611130 China

Research Center of Avian Disease College of Veterinary Medicine Sichuan Agricultural University Chengdu 611130 China

Key Laboratory of Microbial Pathogenesis and Interventions of Fujian Province University the Key Laboratory of Innate Immune Biology of Fujian Province Biomedical Research Center of South China Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education College of Life Sciences Fujian Normal University Fuzhou 350117 China

Funding

National Natural Science Foundation of China · Natural Science Foundation of Sichuan Province · Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System · Earmarked Fund for China Agriculture Research System

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