DataVXAdvanced SciencePaper
journal article·2026-02Open access

One‐Step Coaxial 3D Printing of Pre‐Vascularized Skin Organoid Models with ADSC Microspheres for Enhanced Wound Healing

Kunrui Wang, Xiang Lan, Jianwei Chen, Yu Wu, Delong Zhu, Xiangkai Kong, Ying Hu, Qian Liu, Kun Wang, Tao Xu, Lei Zhu

Advanced Science· Volume 13· Issue 9
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Source abstract

Abstract

Abstract Organoids are important tools for studying organ development, drug screening, and regenerative medicine, yet the absence of integrated vasculature limits their culture and translation. To address this, the PV‐XOM strategy is proposed, which achieves one‐step construction of pre‐vascularized organoids through coaxial bioprinting: the inner phase uses temperature‐responsive sacrificial material and endothelial cells to form hollow vascular channels, while the outer phase is a biomimetic hydrogel matrix containing organoid microspheres. Based on this framework, a pre‐vascularized skin organoid model (PV‐SOM) is established, in which the outer phase is loaded with adipose‐derived stem cell (ADSC) microspheres and skin fibroblasts. In vitro, PV‐SOM achieved rapid vascular closure and maturation; in vivo, it formed abundant neovessels in large skin defects, accelerated wound closure, and improved collagen remodeling. Proteomic analysis further revealed that ADSC microspheres activate the PI3K–AKT–mTOR pathway to regulate vascular formation across multiple stages. These findings show that PV‐XOM offers an effective, scalable solution to the vascularization bottleneck of organoids with strong translational potential.
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Research context

PolymersMaterials processing

OpenAlex topics: 3D Printing in Biomedical Research · Wound Healing and Treatments · Cellular Mechanics and Interactions

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Third Affiliated Hospital of Sun Yat‐Sen University Department of Dermatology and Plastic Surgery Sun Yat‐Sen University No.600 Tianhe Road, Tianhe Guangzhou Guangdong 510630 China

Center for Bio‐Intelligent Manufacturing and Living Matter Bioprinting Research Institute of Tsinghua University in Shenzhen Tsinghua University Shenzhen 518000 China

Department of Research and Development Qingyuan Zhixin (Shenzhen) Biotechnology Co., Ltd Shenzhen Guangdong 518000 China

3D Printing Bio‐Intelligent Manufacturing and Application Engineering Research Center Of Guangxizhuang Autonomous Region Nanning 530021 China

Third Affiliated Hospital of Sun Yat‐Sen University Department of Orthopedic Surgery Sun Yat‐Sen University No.600 Tianhe Road, Tianhe Guangzhou Guangdong 510630 China

Department of Ophthalmology Ninth People's Hospital Shanghai JiaoTong University School of Medicine Shanghai 200011 China

Funding

National Key Research and Development Program of China · Basic and Applied Basic Research Foundation of Guangdong Province

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