Domestication of Tartary Buckwheat Shaped a Regulatory Module for Seedling Salt Tolerance by Targeting the Magnesium Transporter Gene FtMGT2
Source abstract
Abstract
Abstract Globally, soil salinization increasingly affects farmland, severely limiting the production of Tartary buckwheat ( Fagopyrum tataricum ). To identify genetic factors for salt tolerance, we analyzed core Tartary buckwheat accessions and utilized differential expression analysis and genome‐wide association studies (GWAS), identifying a key domesticated magnesium transporter protein, FtMGT2. A single nucleotide polymorphism (SNP) genotype (G/A) of a natural variant located in the FtMGT2 promoter was found to be positively associated with the expression of FtMGT2 and salt tolerance variation. Mechanistically, the MADS transcription factor FtAGL16 binds the A variant more strongly. FtAGL16 and the MYB transcription factor FtMYB15L co‐regulate FtMGT2 transcription, with FtMYB15L protein stability strictly controlled by the E3 ubiquitin ligase FtBRG1. Intriguingly, under salt stress, FtAGL16 can compete with FtBRG1 for binding to FtMYB15L, stabilizing and accumulating FtMYB15L. This enhances FtMGT2 expression, increasing Mg 2+ flux, which in turn enhances the transport activity of the sodium (Na + ) transporter FtHKT1. This coordinated action leads to increased Na + efflux and enhanced salt resistance. This study thereby establishes both the theoretical basis and practical application for targeted molecular breeding to enhance plant salt tolerance.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Magnesium in Health and Disease · Plant Stress Responses and Tolerance · Plant nutrient uptake and metabolism
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
State Key Laboratory of Crop Gene Resources and Breeding/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China
College of Biology and Agriculture Zunyi Normal University Zunyi 563099 China
College of Life Sciences Capital Normal University Beijing 100048 China
College of Agriculture Sichuan Agricultural University Chengdu 611130 China
State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 200237 China
College of Agriculture Guizhou University Guiyang 550025 China
Department of Agronomy Chungbuk National University Cheongju 28644 South Korea
Groupe de Recherche en Physiologie Végétale (GRPV) Earth and Life Institute‐Agronomy (ELI‐A) Université Catholique de Louvain Croix du Sud 4‐5, boîte L7.07.13 Louvain‐la‐Neuve B‐1348 Belgium
Laboratory of Metabolomics Institute of Microbiology Bulgarian Academy of Sciences Plovdiv 4000 Bulgaria
Center of Plant Systems Biology and Biotechnology Plovdiv 4000 Bulgaria
Department of Root Biology and Symbiosis Max‐Planck‐Institute of Molecular Plant Physiology 14476 Potsdam Germany
Funding
National Natural Science Foundation of China · National Key Research and Development Program of China
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