An Opto‐Bio‐Hydrodynamic Platform for Instructing Cardiac Left‐Right Asymmetry Development

Abstract The spatiotemporal regulation of ciliary dynamics within the left‐right organizer (LRO) governs cardiac laterality establishment through biomechanical signaling gradients. While targeted restoration of aberrant ciliary motion can theoretically rescue left‐right patterning defects, existing manipulation strategies lack the non‐invasiveness, micron‐scale precision, and spatiotemporal programmability required for developmental interventions. Here, a multifunctional Opto‐Bio‐Hydrodynamic platform is presented to modulate ciliary motion with the programmable near‐infrared light for instructing cardiac left‐right asymmetry development in a spatiotemporally controlled manner. By the real‐time modulation of a 1064‐nm light beam, a direct optical trapping is demonstrated for the motile cilia, while uniform synchronization of ciliary beating is achieved through indirect optical regulation. Consequently, a coordinated ciliary rotational pattern triggers an enhanced recirculating flow, under which the methylcellulose‐induced cardiogenesis abnormality is rescued in a fully controlled manner. By establishing mechanistic links among optically tuned ciliary dynamics, flow‐mediated signaling, and organ asymmetry, this proposed strategy provides valuable insights into congenital heart defects and offers a promising biomedical platform for developmental bioengineering and mechanobiological therapeutics.