DataVXAdvanced SciencePaper
journal article·2025-06Open access

High‐Throughput Fluorescence Screening Enables Globally Consistent Identification of ABA Signaling Modulators

Yang‐Yang Gao, Chang‐Xin Yang, Hong Wu, Jian‐Hong Li, Ting Wen, Wei Wang, Zhi‐Zheng Wang, Hui‐Min Chen, Rong‐Jie Pei, Zhi‐You Huang, Yu‐Guo Zheng, Guang‐Fu Yang, Xiang‐Yang Li, Ge‐Fei Hao

Advanced Science· Volume 12· Issue 22
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Source abstract

Abstract

Abstract Frequent exposure to environmental stresses can potentially reduce plant yield by up to 60%. The regulation of abscisic acid (ABA) signaling, especially its core receptors, is crucial for the adaptation of plants to environmental stress. However, efficient identification of ABA‐signaling modulators remains a challenge. Here, a global high‐throughput fluorescence screening (GhitFluors) platform is established to efficiently identify ABA signaling modulators. The GhitFluors platform yields a similar tendency for binding affinity to that determined by classical methods. Diopyridin is effectively identified, a regulator with strong binding affinity to Pyrabactin Resistance 1 (PYR1), and more than 80% inhibitory activity against HAB1, which is equivalent to ABA. Additionally, it can also effectively mitigate the adverse effects of drought on plants. The findings promise the start of a new era for achieving high‐efficiency identification ABA signaling modulators.
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Research context

OpenAlex topics: Plant Stress Responses and Tolerance · Plant-Microbe Interactions and Immunity · Plant Molecular Biology Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education Center for Research and Development of Fine Chemicals Guizhou University Guiyang 550025 P. R. China

State Key Laboratory of Biocatalysis and Enzyme Engineering School of Life Sciences Hubei University Wuhan Hubei 430072 P. R. China

State Key Laboratory of Green Pesticide Central China Normal University Wuhan 430079 P. R. China

Key Laboratory of Chemical Synthesis and Environmental Pollution Control Remediation Technology Minzu Normal University of Xingyi Xingyi Guizhou 562400 P. R. China

Funding

National Key Research and Development Program of China · China Postdoctoral Science Foundation · Innovative Research Group Project of the National Natural Science Foundation of China

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