Reinforcing Stromal Cell Spheroid Through Red‐Light Preconditioning for Advanced Vascularization
Source abstract
Abstract
Abstract Despite the promising potential of stromal cell therapy in treating myocardial infarction (MI), its effectiveness is limited by poor cell retention and engraftment in ischemic environments. This study introduces a novel strategy that combines the preconditioning of human adipose‐derived stromal cells (hADSCs) using OLED‐based photobiomodulation (OPBM) and culturing these cells into 3D spheroids. The preconditioned 3D spheroids (APCS group) exhibit significantly enhanced angiogenic, arterialized, and tissue remodeling capabilities compared with those of traditional 2D cultures and non‐preconditioned spheroids. In vivo transplantation of these spheroids into the border zone of infarcted area significantly improve cardiac function and reduce adverse remodeling by enhancing anti‐fibrosis and angiogenesis including arterialization. The combined strategy with OPBM preconditioning and 3D spheroid culture system can enhance therapeutic potential of hADSCs with multiple paracrine effects for cardiac repair. This novel approach provides next generation of cell therapeutics to overcome the limitation of adult stromal cell therapy in patients with post‐MI heart failure.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Angiogenesis and VEGF in Cancer · Tissue Engineering and Regenerative Medicine
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Chemical Engineering Sungkyunkwan University Suwon 16419 Republic of Korea
Center for Biomaterials Biomedical Research Institute Korea Institute of Science and Technology Seoul 02792 Republic of Korea
Department of Biomedicine & Health Sciences College of Medicine The Catholic University of Korea Seoul 06591 Republic of Korea
Department of Biomedical Sciences City University of Hong Kong Hong Kong 999077 China
Department of Molecular Science and Technology Ajou University Suwon 16499 Republic of Korea
Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology & Emory University Atlanta GA 30332 USA
Children's Heart Research & Outcomes (HeRO) Center Children's Healthcare of Atlanta & Emory University Atlanta GA 30322 USA
Division of Cardiology Department of Internal Medicine Uijeongbu St. Mary's Hospital The Catholic University of Korea Seoul 06591 Republic of Korea
Cell Death Disease Research Center College of Medicine The Catholic University of Korea Seoul 06591 Republic of Korea
Funding
Ministry of Science and ICT, South Korea · Korea Evaluation Institute of Industrial Technology
References (82)
Show source-provided references
1. 10.1097/MD.000000000002128810.1097/MD.0000000000021288 ↗
2. 10.3892/etm.2021.1098610.3892/etm.2021.10986 ↗
3. 10.1038/s41569-018-0013-010.1038/s41569-018-0013-0 ↗
4. 10.1038/s41419-020-2542-910.1038/s41419-020-2542-9 ↗
5. 10.1161/CIRCRESAHA.109.20899110.1161/CIRCRESAHA.109.208991 ↗
6. 10.1016/j.jacc.2011.09.06510.1016/j.jacc.2011.09.065 ↗
7. 10.1016/j.ahj.2014.03.02210.1016/j.ahj.2014.03.022 ↗
8. 10.1002/ccd.2660110.1002/ccd.26601 ↗
9. 10.1016/j.jconrel.2021.06.04010.1016/j.jconrel.2021.06.040 ↗
10. 10.1371/journal.pone.013799910.1371/journal.pone.0137999 ↗
11. 10.1152/ajpheart.00688.201710.1152/ajpheart.00688.2017 ↗
12. 10.1186/s10020-019-0128-810.1186/s10020-019-0128-8 ↗
13. 10.1016/j.biomaterials.2014.01.02110.1016/j.biomaterials.2014.01.021 ↗
14. 10.1021/acsnano.7b0100810.1021/acsnano.7b01008 ↗
15. 10.7150/thno.1160710.7150/thno.11607 ↗
16. 10.1002/stem.285310.1002/stem.2853 ↗
17. 10.1007/s13770-023-00547-810.1007/s13770-023-00547-8 ↗
18. 10.1177/2041731421106700410.1177/20417314211067004 ↗
19. 10.1002/btm2.1025510.1002/btm2.10255 ↗
20. 10.1016/j.jphotobiol.2023.11268110.1016/j.jphotobiol.2023.112681 ↗
21. 10.1016/j.apmt.2024.10222310.1016/j.apmt.2024.102223 ↗
22. 10.1186/s41232-022-00226-610.1186/s41232-022-00226-6 ↗
23. 10.1089/photob.2022.012710.1089/photob.2022.0127 ↗
24. 10.1073/pnas.070496610410.1073/pnas.0704966104 ↗
25. 10.1161/JAHA.119.01261710.1161/JAHA.119.012617 ↗
26. 10.1093/cvr/cvw00510.1093/cvr/cvw005 ↗
27. 10.1016/S0008-6363(00)00206-610.1016/S0008-6363(00)00206-6 ↗
28. 10.1161/ATVBAHA.121.31719710.1161/ATVBAHA.121.317197 ↗
29. 10.1038/s41598-021-82284-310.1038/s41598-021-82284-3 ↗
30. 10.1177/14732300090370010810.1177/147323000903700108 ↗
31. 10.1161/01.RES.0000118601.37875.AC10.1161/01.RES.0000118601.37875.AC ↗
32. 10.1557/mrc.2017.8210.1557/mrc.2017.82 ↗
33. Lee D. S. · J. Ind. Eng. Chem. · 2023
34. 10.18632/oncotarget.686810.18632/oncotarget.6868 ↗
35. 10.1038/s41419-019-2155-310.1038/s41419-019-2155-3 ↗
36. 10.1177/194760191142303110.1177/1947601911423031 ↗
37. 10.1016/j.archoralbio.2019.03.00710.1016/j.archoralbio.2019.03.007 ↗
38. Casali B. C. · Biochem. Biophys. Rep. · 2024 · 38
39. 10.1016/j.scr.2021.10242510.1016/j.scr.2021.102425 ↗
40. 10.1002/glia.2407510.1002/glia.24075 ↗
41. 10.4062/biomolther.2015.14610.4062/biomolther.2015.146 ↗
42. 10.1038/s41418-018-0241-110.1038/s41418-018-0241-1 ↗
43. 10.3389/fcell.2020.59057610.3389/fcell.2020.590576 ↗
44. 10.7150/thno.4815210.7150/thno.48152 ↗
45. 10.1161/CIRCRESAHA.110.22213310.1161/CIRCRESAHA.110.222133 ↗
46. 10.1016/j.bbagrm.2019.05.00110.1016/j.bbagrm.2019.05.001 ↗
47. 10.1007/s13770-023-00556-710.1007/s13770-023-00556-7 ↗
48. 10.1038/s41598-018-36063-210.1038/s41598-018-36063-2 ↗
49. 10.4049/jimmunol.160194310.4049/jimmunol.1601943 ↗
50. 10.1161/01.RES.0000193596.94936.2c10.1161/01.RES.0000193596.94936.2c ↗
51. 10.1523/JNEUROSCI.3827-07.200810.1523/JNEUROSCI.3827-07.2008 ↗
52. 10.1097/BOR.0b013e32834b330910.1097/BOR.0b013e32834b3309 ↗
53. 10.3390/cells812155810.3390/cells8121558 ↗
54. 10.1084/jem.2019010310.1084/jem.20190103 ↗
55. 10.1007/s13770-023-00614-010.1007/s13770-023-00614-0 ↗
56. 10.1161/CIRCRESAHA.110.22995510.1161/CIRCRESAHA.110.229955 ↗
57. 10.1161/01.ATV.0000069625.11230.9610.1161/01.ATV.0000069625.11230.96 ↗
58. 10.2217/rme.09.7410.2217/rme.09.74 ↗
59. 10.1016/j.jiec.2023.03.01910.1016/j.jiec.2023.03.019 ↗
60. 10.1136/openhrt-2013-00001610.1136/openhrt-2013-000016 ↗
61. 10.1016/j.cell.2009.05.02410.1016/j.cell.2009.05.024 ↗
62. 10.1016/j.ijcard.2008.04.05910.1016/j.ijcard.2008.04.059 ↗
63. 10.1038/nature1050710.1038/nature10507 ↗
64. 10.1038/s41467-017-00953-210.1038/s41467-017-00953-2 ↗
65. 10.1038/s41467-020-17914-x10.1038/s41467-020-17914-x ↗
66. 10.1093/cvr/cvn02710.1093/cvr/cvn027 ↗
67. 10.1186/s12885-015-1546-910.1186/s12885-015-1546-9 ↗
68. 10.1073/pnas.97.2.52910.1073/pnas.97.2.529 ↗
69. 10.1161/CIRCRESAHA.107.15565510.1161/CIRCRESAHA.107.155655 ↗
70. 10.1152/ajpcell.00346.201610.1152/ajpcell.00346.2016 ↗
71. 10.1161/01.HYP.0000020755.56955.BF10.1161/01.HYP.0000020755.56955.BF ↗
72. 10.1161/CIRCRESAHA.118.31384510.1161/CIRCRESAHA.118.313845 ↗
73. 10.1089/ars.2006.139210.1089/ars.2006.1392 ↗
74. 10.1016/j.jiec.2022.12.01710.1016/j.jiec.2022.12.017 ↗
75. 10.1007/s11373-004-8177-510.1007/s11373-004-8177-5 ↗
76. 10.1016/j.ymthe.2018.10.02010.1016/j.ymthe.2018.10.020 ↗
77. 10.1161/01.RES.0000093984.48643.D710.1161/01.RES.0000093984.48643.D7 ↗
78. 10.3892/ol.2015.403510.3892/ol.2015.4035 ↗
79. 10.1074/jbc.RA118.00187210.1074/jbc.RA118.001872 ↗
80. 10.1126/sciadv.aay699410.1126/sciadv.aay6994 ↗
81. 10.1530/VB-20-000710.1530/VB-20-0007 ↗
82. 10.1002/btpr.303410.1002/btpr.3034 ↗