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journal article·2025-03Open access

Thermally Triggered Double Emulsion‐Integrated Hydrogel Microparticles for Multiplexed Molecular Diagnostics

Eui Ju Jeon, Seungwon Jung, Yoon‐ha Jang, Seoyoung Lee, Song‐Ee Choi, So Young Jeon, Lankyeong Yoon, Bong Kyun Kim, Tae Jong Kim, Kuenyoul Park, Seok Chung, Yong Shin, Sung‐Han Kim, Heungsup Sung, Sang Kyung Kim

Advanced Science· Volume 12· Issue 12
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Source abstract

Abstract

Abstract During the COVID‐19 pandemic, reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) has been recognized as the most reliable diagnostic tool. However, there is a need to develop multiplexed assays capable of analyzing multiple genes simultaneously to expand its application. To address this, a multiplexed RT‐qPCR using a double emulsion (DE)‐based carrier and a polymer microparticle reactor, termed primer‐incorporated network tailored with Taqman probe (TaqPIN) is developed. The DE securely stores nucleic acid reagents like primers and probes within the polymer network until heating releases them for the reaction. The TaqPIN RT‐qPCR demonstrates an amplification efficiency of 93.8% and can detect as few as 20 copies/µL. By loading the multiple microparticles into a single reaction, a multiplexed assay with only one optical channel is enabled. In practice, a nine‐plex assay is designed to distinguish between variants of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Even subtle variations of a single nucleotide can be simultaneously detected. Testing on 75 nasopharyngeal swab samples yields 100% sensitivity and specificity for SARS‐CoV‐2 detection and 94% accuracy in variant discrimination.
Source: crossref · Quality: baseline

Research context

Polymers

OpenAlex topics: Pickering emulsions and particle stabilization · Proteins in Food Systems · Innovative Microfluidic and Catalytic Techniques Innovation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Center for Advanced Biomolecular Recognition Biomedical Research Division Korea Institute of Science and Technology (KIST) Seoul 02792 Republic of Korea

Department of Mechanical Engineering Korea University Seoul 02841 Republic of Korea

Department of HY‐KIST Bio‐convergence Hanyang University Seoul 04763 South Korea

Department of Laboratory Medicine Sanggye Paik Hospital School of Medicine Inje University Seoul 01757 Republic of Korea

Department of Biotechnology College of Life Science and Biotechnology Yonsei University Seoul 03722 Republic of Korea

Department of Infectious Diseases Asan Medical Center University of Ulsan College of Medicine Seoul 05505 Republic of Korea

Department of Laboratory Medicine Asan Medical Center University of Ulsan College of Medicine Seoul 05505 Republic of Korea

KHU‐KIST Department of Converging Science and Technology Kyung Hee University Seoul 02447 Republic of Korea

Funding

National Research Council of Science and Technology · Korea Institute of Science and Technology

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