DataVXAdvanced SciencePaper
journal article·2023-03Open access

Artificial Diploid Escherichia coli by a CRISPR Chromosome‐Doubling Technique

Pengju Wang, Dongdong Zhao, Ju Li, Junchang Su, Chunzhi Zhang, Siwei Li, Feiyu Fan, Zhubo Dai, Xiaoping Liao, Zhitao Mao, Changhao Bi, Xueli Zhang

Advanced Science· Volume 10· Issue 7
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Source abstract

Abstract

Abstract Synthetic biology has been represented by the creation of artificial life forms at the genomic scale. In this work, a CRISPR‐based chromosome‐doubling technique is designed to first construct an artificial diploid Escherichia coli cell. The stable single‐cell diploid E. coli is isolated by both maximal dilution plating and flow cytometry, and confirmed with quantitative PCR, fluorescent in situ hybridization, and third‐generation genome sequencing. The diploid E. coli has a greatly reduced growth rate and elongated cells at 4–5 µm. It is robust against radiation, and the survival rate after exposure to UV increased 40‐fold relative to WT. As a novel life form, the artificial diploid E. coli is an ideal substrate for research fundamental questions in life science concerning polyploidy. And this technique may be applied to other bacteria.
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Research context

OpenAlex topics: CRISPR and Genetic Engineering · Evolution and Genetic Dynamics · Gene Regulatory Network Analysis

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Affiliations

Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin 300308 P. R. China

Key Laboratory of Systems Microbial Biotechnology Chinese Academy of Sciences Tianjin 300308 P. R. China

College of Life Science Tianjin Normal University Tianjin 300382 P. R. China

School of Biological Engineering Dalian Polytechnic University Dalian 116034 P. R. China

Biodesign Center Key Laboratory of Systems Microbial Biotechnology Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin 300308 P. R. China

Funding

National Key Research and Development Program of China · National Natural Science Foundation of China · Natural Science Foundation of Tianjin City

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